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DreamTaq™ Hot Start DNA Polymerase
Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
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| Catalog Number | Color | Quantity |
|---|---|---|
| EP1703 | Colorless | 2500 Units |
| EP1701 | Colorless | 200 Units |
| EP1702 | Colorless | 500 Units |
| EP1711 | Green | 200 Units |
| EP1712 | Green | 500 Units |
| EP1713 | Green | 2500 Units |
| EP1714 | Green | 4 x 2500 Units |
Catalog number EP1703
Price (USD)
1,090.00
Each
Color:
Colorless
Quantity:
2500 Units
Price (USD)
1,090.00
Each
Thermo Scientific DreamTaq Hot Start DNA Polymerase, available in colorless and green formats, is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases. The enzyme is supplied with an optimized 10X DreamTaq Buffer containing magnesium chloride, which eliminates the need for extensive optimization of reaction conditions. DreamTaq Hot Start DNA Polymerase is also available with pre-added density reagent and electrophoresis tracking dyes, as well as in master mix format.
The 10X DreamTaq Green Buffer also includes a density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.
DreamTaq Hot Start DNA Polymerase is formulated to enhance productivity through:
- Better reaction outcomes
- Higher yield of target amplicons from low template amounts
- Increased reaction specificity due to reliable hot-start technology
- Wider range of amplicon lengths with routine amplification of genomic DNA fragments up to 6 kb
- Enhanced convenience
- Room temperature reaction setup
- Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer
- Direct loading of PCR products on a gel with DreamTaq Green Buffer
Applications
- Generation of PCR products for TA cloning
- Routine PCR
- Colony PCR
- Genotyping
- RT-PCR
Specifications
GC-Rich PCR PerformanceLow
PolymeraseDreamTaq DNA Polymerase
Reaction SpeedStandard
Quantity2500 Units
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR
Concentration5 U/μL
Hot StartBuilt-In Hot Start
Overhang3'-A
Reaction FormatSeparate Components
ColorColorless
Unit SizeEach
Contents & Storage
• DreamTaq Hot Start DNA Polymerase (5 U/μL), 5 x 100 μL
• 10X DreamTaq Buffer (with 20 mM MgCl2), 10 x 1.25 mL
Store at -5°C to -30°C.
• 10X DreamTaq Buffer (with 20 mM MgCl2), 10 x 1.25 mL
Store at -5°C to -30°C.