FISH Tag™ DNA Multicolor Kit, Alexa Fluor™ dye combination
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Invitrogen™

FISH Tag™ DNA Multicolor Kit, Alexa Fluor™ dye combination

The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kitRead more
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Catalog number F32951
Price (USD)
714.00
Each
Add to cart
Price (USD)
714.00
Each
Add to cart
The FISH Tag™ DNA Multicolor Kit provides a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. The FISH Tag™ DNA Multicolor Kit contains fluorescent dyes in four colors, making it ideal for simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. We also offer FISH Tag™ Kits in single colors.

FISH Tag™ Kit Specifications:
• Dye (Ex/Em): Alexa Fluor™ 488 (492/520 nm), Alexa Fluor™ 555 (555/565 nm), Alexa Fluor™ 594 (590/615 nm), Alexa Fluor™ 647 (650/670 nm)
• Comprehensive kit includes 16 reagents
• Comes with detailed protocol to help ensure your success


Complete Workflow Solution for FISH
FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor™ dyes. PureLink™ nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade™ Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
FormatKit
Label or DyeAlexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647
Product LineAlexa Fluor™, FISH Tag™
Product TypeDNA In Situ Hybridization Assay Kit
Quantity1 kit
TargetDNA
Target SpecificityNot Target-Specific
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

For imaging, what are the limitations of FISH probes?

FISH probes are generally from 300 to 1500 bp in length. To adequately image a gene or other target, the FISH probes must cover a contiguous length of ˜10 kbp. Shorter strand lengths or low-abundance DNA or RNA targets may be detected with the use of fluorescent amplification methods, such as tyramide signal amplification (TSA). For more information using TSA to amplify FISH detection, see this manual (http://tools.thermofisher.com/content/sfs/manuals/mp20912.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When should I use an RNA FISH probe versus a DNA FISH probe?

RNA FISH probes are the best option for hybridization to mRNA, rRNA, or other RNA targets. DNA FISH probes can also be used to detect RNA targets, but they do not hybridize as well to mRNA and other RNA target molecules. DNA FISH probes are routinely used to detect gene loci on chromosomes. RNA FISH probes can also be used to detect genes on chromosomes, but DNA FISH probes are more robust for this application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.