Invivofectamine™ 3.0 Reagent
Invivofectamine™ 3.0 Reagent
Invitrogen™

Invivofectamine™ 3.0 Reagent

Invivofectamine™ 3.0 Reagent is an animal origin-free lipid nanoparticle designed for high efficiency in vivo delivery of siRNA and miRNARead more
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Catalog NumberNo. of Reactions
IVF300110
IVF300550 Reactions
Catalog number IVF3001
Price (USD)
1,968.00
Each
Add to cart
No. of Reactions:
10
Price (USD)
1,968.00
Each
Add to cart
Invivofectamine™ 3.0 Reagent is an animal origin-free lipid nanoparticle designed for high efficiency in vivo delivery of siRNA and miRNA to mouse liver cells following tail vein injection.

Using Invivofectamine™ 3.0 Reagent
Invivofectamine™ 3.0 Reagent is ideally suited for in vivo delivery of siRNA and miRNA duplexes. Invivofectamine™ 3.0/RNA complexes are delivered using a low-volume, low-pressure method with no toxicity and no stress response in the animal. Invivofectamine™ 3.0 can also be combined with BLOCK-iT™ fluorescent controls to track the biodistribution of the siRNA or miRNA.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ClassificationAnimal Origin-Free
Cell TypeHepatocytes
Sample TypeSynthetic siRNA, siRNA, miRNA, RNAi
Transfection TechniqueLipid-based Transfection, Lipid Nanoparticles, LNPs
SpeciesSmall Animal Models
For Use With (Application)Transfection
No. of Reactions10
Product LineInvivofectamine™
Product TypeReagent
RNAi TypesiRNA, miRNA
Serum CompatibleYes
Shipping ConditionDry Ice
Unit SizeEach

Frequently asked questions (FAQs)

How do I isolate RNA from liver or other organs for my in vivo siRNA experiment?

Isolating high-quality RNA is critical to the overall success of in vivo experiments.

What rate should I use to inject complexed material for a systemic injection for my in vivo siRNA experiment?

Invivofectamine 3.0 Reagent is designed to be delivered slowly and evenly; a 200-µL injection should take between 10 to 20 seconds.

How important is sterile technique for my in vivo siRNA experiment?

Sterile technique is vital to successful completion of in vivo experiments. All materials need to be sterile before injecting the complexed siRNA into animals.

Can I modify the encapsulation protocol to fit my schedule for my in vivo siRNA experiment?

The protocol is robust but specific. Protocol modifications will likely result in less than optimal performance.

What controls should I use for my in vivo siRNA experiment?

Both positive and negative controls are recommended when performing any siRNA experiment. We typically use Invitrogen in vivo siRNA against Factor VII with Invivofectamine 3.0 Reagent, as a positive control for experiments. It is also good practice to use PBS solution as a negative control.

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