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RiboMinus™ Transcriptome Isolation Kit, human/mouse
RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomalRead more
Catalog number K155002
Price (USD)
608.00
Each
Price (USD)
608.00
Each
RiboMinus™ Transcriptome Isolation Kits are novel purification systems that enrich the whole spectrum of RNA transcripts by depleting large ribosomal RNA molecules in a total RNA sample. These kits use a 5μ biotin-labeled, Locked Nucleic Acid (LNA) probe specific for large rRNA to remove 95-98% of rRNA molecules from human, mouse, bacteria, or yeast total RNA (Figure 1). This removal increases the representation of RNA transcript species, including non-poly(A) tailed, pre-processed RNA, small tRNAs, siRNA, snRNA, microRNA, fragmented mRNA, and other regulatory RNAs that cannot be purified via poly(A) selection methods.
The RiboMinus™ Transcriptome Isolation Kits enable:
• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods
• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)
Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.
The RiboMinus™ Transcriptome Isolation Kits enable:
• Comprehensive analysis of the whole transcriptome without contamination or interference from rRNA
• More complete collection of transcribed elements of the genome—no reliance on poly(A) purification methods
• Cleaner, clearer microarray analysis due to higher signal-to-background ratios (Figure 2)
Purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation TechnologyMagnetic Bead
Purification Time1.25 hr.
Sample TypeTotal RNA
Final Product TypeTranscriptome RNA
High-throughput CompatibilityNot High-throughput Compatible (Manual)
For Use With (Application)Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction
Product LineRiboMinus™
Quantity6 preps
Starting Material AmountUp to 10 μg total RNA
TargetTranscriptome RNA
System TypeRiboMinus
Yield1 μg
FormMagnetic Bead
Unit SizeEach
Contents & Storage
The RiboMinus™ Isolation Kits include RiboMinus™ Magnetic Beads, RiboMinus™ human/mouse, bacteria, or yeast probe, and Hybridization Buffer.
The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water.
Store the isolation modules at 4°C, and the concentration module at room temperature.
All reagents are guaranteed stable for 6 months when properly stored.
The optional RiboMinus™ Concentration Module contains spin cartridges, wash tubes, recovery tubes, Binding Buffer, Wash Buffer, and RNase-free (DEPC-treated) water.
Store the isolation modules at 4°C, and the concentration module at room temperature.
All reagents are guaranteed stable for 6 months when properly stored.