Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit
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Invitrogen™

Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit

The Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit is designed for rapid cloning with a Gateway™ entry clone and subsequentRead more
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Catalog number K630001
Price (USD)
762.00
Each
Add to cart
Price (USD)
762.00
Each
Add to cart
The Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit is designed for rapid cloning with a Gateway™ entry clone and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET300/NT-DEST and pET301/CT-DEST, respectively) for convenient purification and detection (Figure 1). The vector kit is ideal for structural biologists who desire no or minimal modifications to their protein of interest. To maximize expression, use with MagicMedia™ E. coli Expression Medium.



Contents and Storage:
The Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit includes 6 μg each of pET300/NT-DEST and pET301/CT-DEST vectors and 10 μg of control vector. Store at -20“C. Guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemInducible
Inducing AgentIPTG
PromoterT7
Product TypeDNA Cloning Vector Kit
Selection Agent (Eukaryotic)None
Antibiotic Resistance BacterialAmpicillin (AmpR)
Protein TagHis Tag (6x)
Cloning MethodGateway
Quantity6 μg
VectorpET, pDEST
Product LineChampion™, Gateway™
Unit SizeEach

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all Champion™ pET300/NT-DEST and pET301/CT-DEST Gateway™ Vector Kit FAQs