MagicMedia™ E. coli Expression Medium
MagicMedia&trade; <i>E. coli</i> Expression Medium
Invitrogen™

MagicMedia™ E. coli Expression Medium

MagicMedia™ E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coliRead more
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Catalog number K6803
Price (USD)
160.00
Each
Add to cart
Price (USD)
160.00
Each
Add to cart
MagicMedia™ E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture O.D. (optical densities) or adding inducer. The proprietary MagicMedia™ formulation enables E. coli growth to reach culture densities 3-10-fold higher than those achieved with traditional LB + IPTG methods, allowing you to:

• Achieve 3-10 times higher protein yields (Figure 1) with much less hands-on time
• Eliminate time-consuming O.D. monitoring steps-simply inoculate prepared MagicMedia™ E. coli Expression Medium, grow the culture overnight, and harvest (Figure 2)
• Reduce culture volume to simplify handling and scale-up processes

In addition, MagicMedia™ E. coli Expression Medium is ideal for simultaneous expression of multiple clones for high-throughput applications.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatLiquid
Product TypeE. Coli Expression Medium
Shipping ConditionRoom Temperature
Target Organism ClassE. coli
Quantity1 L
Product LineMagicMedia™
Unit SizeEach
Contents & Storage
Component A (1X liquid) and Component B (liquid)

Store in the dark at 2−8°C.

Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

Can I use a lower incubation temperature for MagicMedia Medium?

Yes, expression at 18 degrees C and 25 degrees C has been validated using the Dual Temperature expression protocol.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I am using a Champion pET vector with the BL21-AI cells. Can I use your MagicMedia Medium for expression?

Unfortunately, MagicMedia Medium will not work with the arabinose inducer. MagicMedia Medium needs an IPTG-inducible system along with an intact Lac operon.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is MagicMedia Medium?

MagicMedia Medium is an E. coli expression medium. It produces 3-10 times higher protein yield, is compatible with T7 regulated E. coli vectors that contain a functional lac operon and BL21 (DE3) strains, is simple to use, and eliminates OD monitoring/induction steps.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How does expression in MagicMedia affect the solubility of proteins when compared to standard LB and IPTG induction?

In most cases, the ratio of soluble to insoluble proteins is not much different when compared to LB and IPTG. However, since more total protein is typically obtained in MagicMedia, one also obtains more total soluble protein.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How does MagicMedia compare to LB-IPTG with regards to expression of toxic proteins?

Toxic protein expression has not been investigated using MagicMedia. However, the Dual Temperature protocol provided in the manual can be tried. This may be a situation where LB/IPTG induction has an advantage since parameters (time, temperature, and IPTG concentration) are easily adjusted for optimal expression.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.