FreeStyle™ MAX CHO Expression System
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Gibco™

FreeStyle™ MAX CHO Expression System

The FreeStyle MAX System is a complete system for mammalian transient protein production. This system combines Gibco FreeStyle media, FreeStyleRead more
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Catalog number K900020
Price (USD)
2,040.00
Each
Add to cart
Price (USD)
2,040.00
Each
Add to cart
The FreeStyle MAX System is a complete system for mammalian transient protein production. This system combines Gibco FreeStyle media, FreeStyle MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.

The FreeStyle MAX System provides:
• An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
• Up to milligram quantities of functional proteins with the expected post-translational modifications
• Simplified downstream purification of secreted proteins with FreeStyle™ serum-free medium
• Straightforward protocol for large-scale production

The FreeStyle MAX System can replace time-consuming stable cell line generation with large-scale functional protein production in one week.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineFreeStyle™ CHO-S Cells
ClassificationAnimal Origin-Free, Serum-Free
Delivery TypeTransfection
Culture TypeSuspension Cell Culture
Cell TypeCHO
Transfection TechniqueLipid-based Transfection
Expression MechanismCell-Based Expression
Expression SystemMammalian
For Use With (Application)Protein Expression
Key FunctionsProtein Production
Product LineFreestyle™
Product TypeExpression System
Quantity1 kit
Unit SizeEach
Contents & Storage
Store FreeStyle cells in liquid nitrogen. Store FreeStyle MAX Reagent at 2–8°C. Store FreeStyle CHO Expression Medium, FreeStyle 293 Expression Medium, and OptiPRO SFM at 2–8°C in the dark. Store the pCMV SPORT at -20°C. Stability is guaranteed for 6 months under the recommended storage conditions. Please refer to the manual for additional storage information.

Frequently asked questions (FAQs)

I am interested in a mammalian expression system that will allow me to obtain very high yields of my protein. What kind of systems do you offer?

For stable high-yield expression, we offer the Freedom CHO-S Kit (Cat. No. A1369601) and Freedom DG44 Kit (Cat. No. A1373701). For transient high-yield expression, we offer the ExpiCHO Expression System (Cat. No. A29133), Expi293 Expression System (Cat. No. A14635), FreeStyle 293 Expression System (Cat. No. K900001), FreeStyle Max 293 Expression System (Cat. No. K900010), and FreeStyle Max CHO Expression System (Cat. No. K900020). For high-yield expression of functional membrane proteins in Exp293F cells, we offer the Expi293 MembranePro Expression System (Cat. Nos. A25869, A25870) that combines the scalability and ease of use of Expi293 and the technology of MembranePro to allow an increase of more than 20-fold in membrane protein yield compared to the standard, adherent culture MembranePro Functional Protein Expression System. Please see the Application Note (http://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/ProteinExpressionAnalysis/pdfs/PG1391-PJ6257-CO28704-Expi293Adaption-MembraneProTech_AppNoteGlobal-FHR.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What vector do you recommend using with FreeSytle MAX CHO Expression System (Cat. No. K900020)?

We recommend using the pcDNA 3.4 TOPO TA Cloning Kit (Cat. No. A14697).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.