LC3B Antibody Kit for Autophagy
LC3B Antibody Kit for Autophagy
Invitrogen™

LC3B Antibody Kit for Autophagy

The LC3 Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use inRead more
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Catalog number L10382
Price (USD)
534.00
Each
Add to cart
Price (USD)
534.00
Each
Add to cart
The LC3 Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use in fluorescence microscopy and high content imaging and analysis. Chloroquine diphosphate is also included - following treatment with this compound, normal autophagic flux is disrupted and autophagosomes accumulate as a result of the disruption in normal autophagic flux.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Equipment)Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument
Product TypeDye
Dye TypeUnlabeled
Format96-well plate, Slide(s)
Quantity1 kit
Shipping ConditionDry Ice
Unit SizeEach
Contents & Storage
Kit includes includes a rabbit polyclonal antibody against LC3B and chloroquine diphosphate.
  • Store at ≤ -20°C

    • Frequently asked questions (FAQs)

    How does the LC3B Antibody Kit for Autophagy work?

    The LC3B protein plays a critical role in autophagy. This protein resides in the cytosol, but following cleavage and lipidation with phosphatidylethanolamine, LC3 associates with the phagophore. This localization can be used as a general marker for autophagic membranes. The LC3B Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use in fluorescence microscopy and high content imaging and analysis. The kit also includes chloroquine diphosphate for artificially generating autophagosomes. Following chloroquine diphosphate treatment, lysosomal pH increases and the normal autophagic flux is disrupted, resulting in autophagosome accumulation.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.