Lipofectamine™ 3000 Transfection Reagent
Try Lipofectamine 3000 Transfection Reagent for free with the Aspire member program
New Transfection Support Center—find technical tips and troubleshooting recommendations from our scientists.
Lipofectamine™ 3000 Transfection Reagent
Invitrogen™

Lipofectamine™ 3000 Transfection Reagent

Green features
Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomesRead more
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
L30000010.1 mL
L30000080.75 mL
L30000151.5 mL
L30000755 x 1.5 mL
L300015015 mL
Catalog number L3000001
Price (USD)
87.50
Each
Add to cart
Quantity:
0.1 mL
Price (USD)
87.50
Each
Add to cart
Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

• Superior performance—our highest efficiency reagent for difficult-to-transfect cells
• Improved cell viability—gentle on your cells, with low toxicity
• Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine™ 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)
Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)
Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)
Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)
Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)
Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)

View performance data for 60+ cell lines plus comparison to Lipofectamine 2000 >

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine™ 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5 mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75 μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine™ 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ClassificationAnimal Origin-Free
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity0.1 mL
Serum CompatibleYes
Unit SizeEach
Contents & Storage
Contains one vial of Lipofectamine™ 3000 Reagent and one vial of P3000TM Enhancer Reagent. Store at 2–8°C. Do not freeze.

Frequently asked questions (FAQs)

I accidentally left my Lipofectamine 3000 Transfection Reagent at room temperature. Can I still use it?

Yes, Lipofectamine 3000 Transfection Reagent is stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

I accidentally froze my Lipofectamine 3000 Reagent. Can I still use it?

Freezing can alter the integrity and composition of the lipid particles. The performance of the reagent may be affected and it may only work for the first week or so. It is safer to use a new vial.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Can I use Lipofectamine RNAiMAX to co-transfect siRNA with plasmid DNA?

While Lipofectamine 3000 or Lipofectamine 2000 may be used for co-transfection of siRNA with plasmid DNA, Lipofectamine RNAiMAX cannot be used.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use Lipofectamine MessengerMAX Transfection Reagent to deliver plasmid DNA?

Yes, Lipofectamine MessengerMAX Transfection Reagent can deliver plasmid DNA in combination with mRNA (i.e., in CRISPR); however, Lipofectamine 3000 Transfection Reagent is best optimized for plasmid DNA delivery and superior DNA transfection performance.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.