SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
SimplyBlue™ SafeStain
Invitrogen™

SimplyBlue™ SafeStain

Green features
SimplyBlue SafeStain is a ready-to-use, fast, sensitive, and safe Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels and on dry PVDF membranes.
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Catalog NumberDescription
LC6060SimplyBlue™ SafeStain, 1 L
LC6065SimplyBlue™ SafeStain, 3.5 L
Catalog number LC6060
Price (USD)
218.00
Each
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Description:
SimplyBlue™ SafeStain, 1 L
Request bulk or custom format
Price (USD)
218.00
Each
Add to cart
SimplyBlue SafeStain is a ready-to-use, fast, sensitive, and safe Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels and on dry PVDF membranes. SimplyBlue SafeStain is completely non-hazardous and does not require methanol or acetic acid fixatives or destains. Risk of hazardous exposure and unpleasant odors are eliminated, creating a healthier lab environment.
Coomassie G-250 stain for visualizing protein bands on polyacrylamide gels and on dry PVDF membranes. SimplyBlue SafeStain is completely non-hazardous and does not require methanol or acetic acid fixatives or destains. Risk of hazardous exposure and unpleasant odors are eliminated, creating a healthier lab environment.

Features include:
• An easy-to-perform protocol that can be completed in less than three hours. For added speed, a simple microwave procedure can be completed in 12 minutes
• Destaining not required but may be performed with deionized water to achieve maximum sensitivity, especially when performing downstream analyses such as mass spectrometry, or when a crystal-clear background is required
• Sensitive—detect down to 7 ng of protein
• Fast—detect 1 μg of protein in as little as five minutes after staining
• Convenient—save valuable time by skipping reagent preparation with this ready-to-use formulation
• Non-hazardous—save money and hassle associated with hazardous shipping and disposal of hazardous waste

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionSimplyBlue™ SafeStain, 1 L
Detection LocationIn-Gel Detection
Detection MethodColorimetric
Label or DyeCoomassie
Product TypeSafe Protein Stain
Shelf Life6 Months
Green FeaturesLess hazardous
Product LineSimplyBlue™
Quantity1 L
Target MoleculeProtein
Unit SizeEach
Contents & Storage
SimplyBlue™ SafeStain is supplied as a 1X ready-to-use staining reagent. Store at room temperature. Guaranteed stable for 6 months when properly stored. There are no HazMat charges associated with this product.

Frequently asked questions (FAQs)

Can membranes be stained with the Colloidal Blue (G-250) Staining Kit after blotting?

No we do not recommend staining membranes with the Colloidal Blue (G-250) Staining Kit as the background will be too high. Better alternatives include:

- Invitrogen Reversible Membrane Protein Stain (Cat. No. IB7710): Allows for complete, reversible staining of protein on nitrocellulose & PVDF membranes. Sensitivity is higher than Ponceau S (<10 ng of BSA in 10 mins as blue bands) and the staining is reversible in 5 minutes. Western blot detection is unaffected by the staining and erasing process, and in some cases higher sensitivity is achieved.
- Coomassie (non-colloidal) staining: Stain in 0.1% Coomassie Blue R-250 in 50% methanol for 5 minutes and destain with several changes of 50% methanol and 10% acetic acid. Rinse with several changes of water, air dry and store for up to 12 months at −20 degrees C. Sensitivity is approximately at the 50-100 ng level.
- SimplyBlue SafeStain. There is a protocol included in the SimplyBlue SafeStain manual for staining dry PVDF membranes but it is not recommended for wet PVDF membranes or nitrocellulose because of the high background.
- Amido black: same as Coomassie stain but less sensitive. Please see protocol in the manual (https://tools.thermofisher.com/content/sfs/manuals/invitrolonpvdf_man.pdf).
- Ponceau S: same as Coomassie stain but less sensitive. Please see protocol in the manual (https://tools.thermofisher.com/content/sfs/manuals/invitrolonpvdf_man.pdf).
- UV transillumination: After blotting, place the membrane on a filter paper and allow to air-dry at room temperature for about 10 minutes. Rewet in 20% methanol and view the blot in front of white light while it is still wet; the bands will look more translucent than the membrane. If the bands disappear because the membrane is dry, rewet the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I stain my gel with Colloidal Blue Stain and then perform western blotting?

Yes, Colloidal Blue Stain can be used before western blotting. However, for optimal transfer efficiency, we recommend destaining the gel and then equilibrating in a series of Tris base/Glycine/SDS solutions to increase solubility; when the transfer is complete, the membrane should be treated with methanol to remove the stain prior to chromogenic development (not necessary prior to chemiluminescent detection).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.