SilverXpress™ Silver Staining Kit
SilverXpress™ Silver Staining Kit
Invitrogen™

SilverXpress™ Silver Staining Kit

The SilverXpress Silver Staining Kit is a highly sensitive silver staining kit providing nanogram-level sensitivity with minimal background. The easy-to-followRead more
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Catalog number LC6100
Price (USD)
408.00
Each
Add to cart
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Price (USD)
408.00
Each
Add to cart
The SilverXpress Silver Staining Kit is a highly sensitive silver staining kit providing nanogram-level sensitivity with minimal background. The easy-to-follow protocol requires less total time than standard Coomassie staining and is complete in a little over one hour.

Compare all silver stains ›

The SilverXpress Silver Staining Kit uses ammoniacal silver chemistry and glutaraldehyde sensitization to produce a highly sensitive silver stain, capable of detecting much lower levels of protein than standard Coomassie or Colloidal Blue techniques. Clear background makes sample identification unambiguous and provides publication-quality gels.

DNA staining
The SilverXpress kit may also be used for detecting nanogram concentrations of DNA that cannot be detected using ethidium bromide. It is five-times more sensitive than ethidium bromide, detecting as little as less than 0.5 ng of 50 bp DNA.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodColorimetric
Label or DyeSilverXpress Silver Stain
Product TypeProtein Gel Staining Kit
Shelf Life6 Months
Product LineSilverXpress™
Quantity1 kit
Shipping ConditionRoom Temperature
Target MoleculeProtein
Unit SizeEach
Contents & Storage
This kit contains sufficient reagents to stain 25 minigels

Contains:
125 mL Sensitizer
125 mL Stainer A
125 mL Stainer B
125 mL Developer
125 Stopper solution

Store kit at 2°C to 8°C. Stable for 6 months when stored under these conditions.

Frequently asked questions (FAQs)

Can I stain my TBE gel? How?

Yes, you can stain your TBE gels with ethidium bromide, SYBR Green I, SYBR Green II, and the SilverXpress Silver Staining Kit. For ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light.

If you need to stop in the middle of the SilverQuest or SilverXpress silver staining procedure, at what point should this be done?

The sensitizing step is the optimal point to stop the procedure. The gel can be left overnight in the Sensitizing Solution. Although some other kits recommend leaving gels in the fix step, we have found that overnight fixation diminishes stain performances.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of Coomassie staining and silver staining?

Coomassie sensitivity: 50-500 ng protein per band
Silver-staining sensitivity: 1-5 ng protein per band
In general, silver staining is 10-100 times more sensitive than Coomassie staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverXpress Silver Staining kit and my stained gels are too dark. What went wrong?

Here are possible causes and solutions:

- Stopper not added to the gel at the appropriate time. Be sure to add the stopper slightly before the desired stain intensity is reached.
- Protein is overloaded. Decrease the protein load on the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverXpress Silver Staining kit and I see negative staining (a dark halo enclosing a pale area). Can you please help me troubleshoot?

This is usually due to overloading of the protein sample. We recommend decreasing the protein load per band. For an unknown protein, a serial dilution may be necessary to determine the best amount to load for a particular protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.