Novex™ TBE Running Buffer (5X)
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Novex™ TBE Running Buffer (5X)
Invitrogen™

Novex™ TBE Running Buffer (5X)

Novex™ TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10Read more
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Catalog number LC6675
Price (USD)
58.75
Each
Add to cart
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Price (USD)
58.75
Each
Add to cart
Novex™ TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex™ DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex™ TBE and DNA Retardation Gels are designed to run on the XCell SureLock™ Mini-Cell.

Formulation: Novex™ TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended Buffers: For optimum performance, Novex™ TBE Running Buffer and Novex™ Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex™ Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferRunning Buffer
Concentration5 X
Gel CompatibilityPolyacrylamide Gels, Agarose Gels
For Use With (Application)Nucleic Acid Gel Electrophoresis, Blotting
Product LineNovex™
Product TypeTBE Running Buffer
Quantity1 L
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store at room temperature

Frequently asked questions (FAQs)

My Novex TBE buffer has precipitated out of solution. What can I do?

If a slight turbidity develops, the fine precipitate can be dissolved by autoclaving for 5 minutes at 110°C. Do not autoclave in the container supplied. This treatment has no deleterious effect on the buffering properties of TBE.

Do you have the recipe for the TBE running buffer and the Hi-Density TBE Sample Buffer?

Please see the recipes below:

TBE Running Buffer (5X):
Tris base, 54.0 g
Boric acid, 27.5 g
EDTA (free acid), 2.9 g
Deionized water to 1.0 L

Hi-Density TBE Sample Buffer (5X):
5X TBE Running buffer (Cat. No. LC6675), 2 mL
Ficoll Type 400, 1.5 g
Bromophenol blue, 1 mL of 1% solution
Xylene cyanol 1 mL of 1% solution
Deionized water to 10.0 mL