Lipofectamine™ MessengerMAX™ Transfection Reagent
Lipofectamine™ MessengerMAX™ Transfection Reagent
Invitrogen™

Lipofectamine™ MessengerMAX™ Transfection Reagent

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Lipofectamine™ MessengerMAX mRNA Transfection Reagent delivers amazing transfection efficiency in neurons and a broad-spectrum of primary cells, enabling improved applicationRead more
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Catalog NumberQuantity
LMRNA0080.75 mL
LMRNA0010.1 mL
LMRNA0030.3 mL
LMRNA0151.5 mL
LMRNA15015 mL
Catalog number LMRNA008
Price (USD)
700.00
Each
Add to cart
Quantity:
0.75 mL
Price (USD)
700.00
Each
Add to cart
Lipofectamine™ MessengerMAX mRNA Transfection Reagent delivers amazing transfection efficiency in neurons and a broad-spectrum of primary cells, enabling improved application outcomes and more biologically relevant research. That's because our novel lipid nanoparticle technology is optimized to deliver the highest amount of mRNA possible without the nuclear entry step that is required with DNA.

Successfully transfect more predictive cell models with a reagent that provides:
• Amazing transfection efficiency in neurons and primary cell types
• Faster protein expression with no risk of genomic integration
• Up to 10x higher cleavage using mRNA CRISPRs

Enable high transfection efficiency in novel genome editing applications
Lipofectamine™ MessengerMAX™ reagent helps increase the likelihood of successful cleavage and recombination with GeneArt™ CRISPR Nuclease mRNA through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Efficiently transfect difficult-to-transfect cells
Lipofectamine™ MessengerMAX™ reagent is designed to transfect a higher amount of mRNA into neurons and a broad spectrum of difficult-to-transfect primary cells. This eliminates the need for electroporation or viruses, and provides a > 2-fold improvement in transfection efficiency compared to other lipid based reagents.

Leverage advantages of mRNA transfection
Transfection of mRNA does not require nuclear uptake, which results in faster protein expression than DNA transfection since translation of mRNA occurs in the cytoplasm. Also, mRNA has no risk of genomic integration and transfection efficiency is cell cycle independent.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEstablished Cell Lines, Neuronal Cells, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypemRNA
Transfection TechniqueLipid-based Transfection
For Use With (Application)Transfection
Green FeaturesSustainable packaging
Product LineLipofectamine™
Product TypeTransfection Reagent
Quantity0.75 mL
Serum CompatibleYes
Shipping ConditionRoom Temperature
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C).

Frequently asked questions (FAQs)

Can I deliver single-stranded oligonucleotides with Lipofectamine MessengerMAX Transfection Reagent?

If you are delivering a single-stranded oligonucleotide on its own, we recommend using Lipofectamine RNAiMAX Transfection Reagent. However, Lipofectamine MessengerMAX Transfection Reagent can be used for genome editing purposes when Cas9 mRNA is co-delivered with a sgRNA and a single-stranded or double-stranded donor template.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How do I use Lipofectamine MessengerMAX Reagent to deliver both my GeneArt Cas9 mRNA and IVT gRNA together?

Lipofectamine MessengerMAX Reagent may be used for both single gRNA delivery or multiplexing (gRNA for multiple targets) purposes with high transfection efficiency when used with GeneArt Cas9 mRNA. For a detailed protocol, please refer to the GeneArt Cas9 mRNA manual (https://www.thermofisher.com/order/catalog/product/A25640?ICID=search-a25640). We recommend using either the Invitrogen GeneArt Genomic Cleavage Detection Kit (Cat. No. A24732) or Invitrogen GeneArt Genomic Cleavage Selection Kit (Cat. No. A27663) for mutant analysis.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Which cell types can be used with Lipofectamine MessengerMAX Transfection Reagent?

Lipofectamine MessengerMAX Reagent demonstrates low toxicity and high transfection efficiency for all cell types (easy or difficult-to-transfect, primary, and stem cells).

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

When would I use Lipofectamine MessengerMAX Reagent over other transfection reagents?

Lipofectamine MessengerMAX Regent is an excellent reagent for co-delivery of Invitrogen GeneArt CRISPR Cas9 mRNA with in vitro transcribed gRNA for geneome editing purposes. Lipofectamine MessengerMAX Reagent has the added benefit of flexibly delivering short dsDNA or HDR templates (0.5-1 kb) which can be ordered through the Invitrogen GeneArt Strings services.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the Invitrogen Lipofectamine MessengerMAX Transfection Reagent?

Lipofectamine MessengerMAX Transfection Reagent is an animal origin-free transfection reagent, especially formulated for the delivery of mRNA, small RNA (eg.,CRISPR IVT gRNA, siRNA, or miRNA), and short dsDNA or HDR templates (0.5-1 kb). Lipofectamine MessengerMAX Reagent is an excellent reagent choice for CRISPR-mediated genome editing applications.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.