Neon™ Transfection System 100 μL Kit
The Neon Transfection instrument (Cat. No. MPK5000 and MPK5000S) has been discontinued. The Neon consumables (Cat. No. MPK1025, MPK10025, MPK1096, MPK10096, and MPT100) will be discontinued on September 30, 2025 or while supplies last. Learn about the benefits of the new Neon NxT Electroporation System with improved usability, more protocols with plate setup, and connectivity.
Neon™ Transfection System 100 μL Kit
Invitrogen™

Neon™ Transfection System 100 μL Kit

The Neon™ Transfection System 100 μL Kit is designed specifically for use with the Neon™ Transfection System. Use this kitRead more
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Catalog NumberNo. of Reactions
MPK1009696 x 2
MPK1002550 Reactions
Catalog number MPK10096
Price (USD)
2,295.00
Each
Add to cart
No. of Reactions:
96 x 2
Price (USD)
2,295.00
Each
Add to cart
The Neon™ Transfection System 100 μL Kit is designed specifically for use with the Neon™ Transfection System. Use this kit for transfection volumes of 100 μL, containing 5 × 105–2 × 106 adherent cells or 1 × 106–5 × 106 suspension cells. Cells that have been transfected using the included 100 μL Neon™ Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ClassificationAnimal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Growth Factors, Reduced Serum
Cell TypeStem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA
Transfection TechniqueElectroporation
Concentration5 x 10^5 - 2 x 10^6 cells/mL (Adherent Cells), 1 x 10^6 - 5 x 10^6 cells/mL
For Use With (Equipment)Neon™ Transfection System
For Use With (Application)Transfection
No. of Reactions96 x 2
Product LineNeon™
Product TypeTransfection System Kit
Quantity96 x 2 Reactions
Serum CompatibleYes
Shipping ConditionRoom Temperature
Starting Material Amount2x10^4 - 6 x10^8 cells; Varies by cell type
System TypeNeon™ Transfection System
Volume (Metric) Sample100μl
Unit SizeEach
Contents & Storage
• 30 mL Resuspension Buffer R
• 30 mL Resuspension Buffer T
• 2 x 150 mL Electrolytic Buffer E2
• 96 x 100 μL Neon™ Tips
• 20 Neon™ Electroporation Tubes

Store the buffer box 4°C and the tips & tubes box at room temperature.

Frequently asked questions (FAQs)

Do you have suggestions for improving viability when transfecting HL-60 cells with the Neon system?

Our online protocol suggests to use 50,000 cells with the 10 microliter tip. However, HL-60 cells do not survive well when transfected at low density. To improve viability you can try using at least 100,000 (better: 200,000) cells with the 10 microliter tips. Also, make sure your plasmid DNA is highly purified, as HL-60 cells are sensitive to LPS. If you observe that viability is good after 24 hours but decreases over the next 72 hours, you may either be using the wrong culture medium (RPMI 1640 + 10% FBS is recommended, do not use DMEM) or your batch of FBS contains low levels of cytotoxic contaminants.

Other possibilities are mycoplasma contamination or very high passage number of your cells. If this is the case please buy a fresh vial of HL-60 cells from ATCC and try your transfections again.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.