AmpliTaq™ DNA Polymerase with Buffer I or Buffer II
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AmpliTaq™ DNA Polymerase with Buffer I or Buffer II
Applied Biosystems™

AmpliTaq™ DNA Polymerase with Buffer I or Buffer II

AmpliTaq DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli.
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Catalog NumberIncludesQuantity
N8080153Buffer II3000 Units
N8080161Buffer II250 Units
N8080172Buffer II1000 Units
N8080156Buffer II5000 Units
N8080186Buffer II25,000 units
N8080152Buffer I3000 Units
N8080155Buffer I5000 Units
N8080160Buffer I250 Units
N8080171Buffer I1000 Units
N8080185Buffer I25,000 Units
Catalog number N8080153
Price (USD)
1,432.00
Each
Add to cart
Includes:
Buffer II
Quantity:
3000 Units
Request bulk or custom format
Price (USD)
1,432.00
Each
Add to cart
AmpliTaq DNA Polymerase is a 94-kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process, and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial and lot-to-lot.

AmpliTaq DNA Polymerase is available with GeneAmp10X PCR Buffer I or with GeneAmp 10X PCR Buffer II and MgCl2 Solution.

Reliable and robust PCR

The thermal activity profile of AmpliTaq DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ∼40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications.

Features

  • The most thoroughly characterized enzyme available for the PCR process
  • Excellent thermal activity profile makes it good for PCR applications
  • QC-tested to guarantee reproducible results

Notes

  • For superior PCR performance, we recommend DreamTaq DNA Polymerase.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatStand-alone enzyme
PolymeraseAmpliTaq DNA Polymerase
Reaction SpeedStandard
Exonuclease Activity5' - 3'
Product TypeDNA Polymerase
Quantity3000 Units
Shipping ConditionDry Ice
For Use With (Application)Standard PCR
Concentration5 U/μL
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions2400 Reactions
Overhang3'-A
Reaction FormatSeparate Components
Size (Final Product)5 kb or less
Starting MaterialDNA
IncludesBuffer II
Unit SizeEach
Contents & Storage
• AmpliTaq DNA Polymerase (5 U/μL), 12 x 50 μL
• GeneAmp 10X PCR Buffer II, 12 x 1.5 mL
• 25 mM MgCl2, 12 x 1.5 mL

Store at -20°C.

Frequently asked questions (FAQs)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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