NanoDrop™ 3300 Fluorospectrometer
NanoDrop™ 3300 Fluorospectrometer
Actual product may vary
NanoDrop™ 3300 Fluorospectrometer
NanoDrop™ 3300 Fluorospectrometer
Thermo Scientific™

NanoDrop™ 3300 Fluorospectrometer

Choose this versatile, cost-effective fluorospectrometer for broad spectrum fluorescence analysis.
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Catalog NumberModelLampFor Use With (Application)
ND-3300NanoDrop 3300 FluorospectrometerLight emitting diodesNucleic Acid Quantification, DNA Quantification, RNA Quantification, Protein Quantification
ND3300LAPTOPNanoDrop 3300 Fluorospectrometer with Laptop Computer3 light emitting diodes (LEDs)Nucleic acid, protein and custom fluorophores
Catalog number ND-3300
Price (USD)
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Model:
NanoDrop 3300 Fluorospectrometer
Lamp:
Light emitting diodes
For Use With (Application):
Nucleic Acid Quantification, DNA Quantification, RNA Quantification, Protein Quantification

New sales of this model have been discontinued. If you're using fluorescence technology in your workflows, please consider the Invitrogen™ Qubit™ 4 Fluorometer for accurate, specific, sensitive, simple, and fast quantitation. For the latest advances in microvolume UV-Vis technology, the Thermo Scientific™ NanoDrop™ One/OneC models offer enhanced capabilities such as distinguishing mammalian DNA and RNA in nucleic acid samples and software that supports compliant use in 21 CFR Part 11 regulated labs.

New sales of this model have been discontinued. If you’re using fluorescence technology in your workflows, please consider the Invitrogen™ Qubit™ 4 Fluorometer for accurate, specific, sensitive, simple, and fast quantitation. For the latest advances in microvolume UV-Vis technology, the Thermo Scientific™ NanoDrop™ One/OneC models offer enhanced capabilities such as distinguishing mammalian DNA and RNA in nucleic acid samples and software that supports compliant use in 21 CFR Part 11 regulated labs.
 

Compatible with:

Professional versions of Microsoft™ Windows™ 7 (32 and 64 bit), Professional version of Windows 8.1 (64 bit), Windows 10 Pro (64 bit)

US Patents 6,628,382 and 6,809,826.
Specifications
DescriptionND-3300
ModelNanoDrop 3300 Fluorospectrometer
Certifications/ComplianceUL/CSA and CE
Connection TypeUSB
Sample Volume1 μL
Detector Type2048-element linear silicon CCD array
Excitation Wavelength365 nm (UV), 470 nm (Blue), 460 to 650 nm (White)
Product LineNanoDrop
TypeFluorospectrometer
Wavelength Range400 to 750 nm
For Use With (Application)Nucleic Acid Quantification, DNA Quantification, RNA Quantification, Protein Quantification
LampLight emitting diodes
Wavelength Accuracy±1nm
Width (Metric)14 cm
Depth (Metric)20 cm
Weight (English)3.3 lb
Weight (Metric)1.5 kg
Voltage5 VDC
Unit SizeEach

Frequently asked questions (FAQs)

What additional analytes or biomolecules are routinely analyzed using the NanoDrop 3300 Fluorospectrometer?

The NanoDrop 3300 is routinely used for the following:
- Determine the concentration of nucleic acids (some of the pre-configured methods are dsDNA Hoechst dye, dsDNA PicoGreen dye and RNA RiboGreen dye)
- Characterize molecular beacon probes labeled with different fluorophores
- Assess the fluorescence of dyes such as Alexa, Cy and DyLightdyes
- Measure fluorescence of a wide variety of inherent fluorophores

Can I simply wipe the pedestal surface, of the NanoDrop 3300 Fluorospectrometer, enough to prevent carryover?

Yes, we recommend using dry laboratory wipes as a very effective method for removing the sample. The highly polished quartz and stainless-steel surfaces of the sample retention system are resistant to sample adherence.

What is the dynamic range for proteins that can be measured on the NanoDrop 3300 Fluorospectrometer?

Using a 2 µL sample, the range using the Quant-iT protein assay is 0.01 µg - 1.0 µg (BSA).
The range using the fluorescein assay is > 4 logs.
Detailed performance data using other fluorophores may be found on the Thermo Fisher website.

Is the NanoDrop 3300 Fluorospectrometer affordable, even though it allows for a large amount of flexibility?

The NanoDrop 3300 Fluorospectrometer is very affordable compared to high-end fluorescence instruments, primarily because the NanoDrop 3300 Fluorospectrometer does not use costly monochromators.

Can I use the NanoDrop 3300 Fluorospectrometer if I do not know what excitation source to use for my sample?

The Fluorescence Profiler feature, in the NanoDrop 3300 Fluorospectrometer, allows LED excitation selection guidance when you are unsure of the excitation source and/or emission profile of a specific or unknown fluorophore.