NuPAGE™ Sample Reducing Agent (10X)
NuPAGE™ Sample Reducing Agent (10X)
Invitrogen™

NuPAGE™ Sample Reducing Agent (10X)

NuPAGE Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains 500 mM dithiothreitolRead more
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Catalog NumberQuantity
NP000910mL
NP0004250μL
Catalog number NP0009
Price (USD)
79.25
Each
Add to cart
Quantity:
10mL
Price (USD)
79.25
Each
Add to cart
NuPAGE Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains 500 mM dithiothreitol (DTT) for a 10X concentration in a stabilized liquid form.

See all available buffers and reagents available for SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration500 mM
Product TypeSample Reducing Agent
Shelf Life6 Months
Product LineNuPAGE™
Quantity10mL
Shipping ConditionWet Ice
Unit SizeEach
Contents & Storage
NuPAGE™ Sample Reducing Agent (500 mM dithiothreitol) should be stored at 4°C. Guaranteed stable for 6 months unless otherwise specified in the product literature.

Frequently asked questions (FAQs)

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can other reducing agents other than DTT or BME be used to reduce proteins prior to electrophoresis? For example, what about TCEP (Tris Carboxy Ethyl Phosphene)?

TCEP, Tris Carboxy Ethyl Phosphene is an alternative sulfhydryl reducing agent for protein samples. It is an extremely potent and effective reducing agent for particularly ‘difficult' proteins. It is compatible with the Tris-Glycine gels and NuPAGE gels. It should be added to the sample buffer for these systems. 20 mM final (maximum) concentration is sufficient for samples. You may add alkylating agents, e.g. Iodine (50 mM Iodoacetic acid), to prevent re-forming of S-S bonds but it is not necessary. Do not heat because this will hydrolyze much of your sample. Instead let the sample sit for several minutes at RT and then load.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.