NuPAGE™ Bis-Tris Mini Protein Gels, 12%, 1.0 mm
Please note that at certain times of the year Bis-Tris protein gel boxes may arrive inside insulated coolers containing ambient temperature gel packs.
NuPAGE™ Bis-Tris Mini Protein Gels, 12%, 1.0 mm
NuPAGE™ Bis-Tris Mini Protein Gels, 12%, 1.0 mm
NuPAGE™ Bis-Tris Mini Protein Gels, 12%, 1.0 mm
Invitrogen™

NuPAGE™ Bis-Tris Mini Protein Gels, 12%, 1.0 mm

NuPAGE™ Novex™ 12% Bis-Tris Gels are pre-cast polyacrylamide gels designed to give optimal separation of small- to medium-sized proteins under denaturing conditions.
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Catalog NumberWellsQuantity
NP0341BOX10-well10 Gels/Box
NP0341PK210-well2 Gels/Box
NP0342BOX12-well10 Gels/Box
NP0342PK212-well2 Gels/Box
NP0343BOX15-well10 Gels/Box
NP0344BOX1-well10 Gels/Box
NP0346BOX2D-well10 Gels/Box
NP0349BOX17-well10 Gels/Box
Catalog number NP0341BOX
Price (USD)
164.00
Each
Add to cart
Wells:
10-well
Quantity:
10 Gels/Box
Price (USD)
164.00
Each
Add to cart
NuPAGE™ Novex™ 12% Bis-Tris Gels are pre-cast polyacrylamide gels designed to give optimal separation of your small- to medium-sized proteins under denaturing conditions. Unlike traditional Tris-Glycine gels, NuPAGE™ Novex™ Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE™ Novex™ Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer (NP0007) and NuPAGE Sample Reducing Agent (NP0004). Use NuPAGE Antioxidant (NP0005) in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer (NP0002) to better resolve small proteins or NuPAGE MOPS SDS Running Buffer (NP000102) to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer (NP00061) for traditional wet transfer using the Mini Blot Module (B1000) or XCell II Blot Module (EI9051). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB210010).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 μL
Separation Range3.5 to 80 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit SizeEach

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What does it mean when bands appear to be getting narrower (or "funneling") as they progress down a protein gel?

There may be too much beta-mercaptoethanol (BME), sample buffer salts, or dithiothreitol (DTT) in your samples. If the proteins are over-reduced, they can be negatively charged and actually repel each other across the lanes causing the bands to get narrower as they progress down the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.