Bolt™ Bis-Tris Plus Mini Protein Gels, 10%, 1.0 mm, WedgeWell™ format
Please note that at certain times of the year Bis-Tris protein gel boxes may arrive inside insulated coolers containing ambient temperature gel packs.
Bolt™ Bis-Tris Plus Mini Protein Gels, 10%, 1.0 mm, WedgeWell™ format
Bolt™ Bis-Tris Plus Mini Protein Gels, 10%, 1.0 mm, WedgeWell™ format
Bolt™ Bis-Tris Plus Mini Protein Gels, 10%, 1.0 mm, WedgeWell™ format
Invitrogen™

Bolt™ Bis-Tris Plus Mini Protein Gels, 10%, 1.0 mm, WedgeWell™ format

Similar to NuPAGE™ Bis-Tris gels, Bolt™ Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications.
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Catalog NumberWells
NW00100BOX10-well
NW00102BOX12-well
NW00105BOX15-well
NW00107BOX17-well
Catalog number NW00100BOX
Price (USD)
178.00
Each
Add to cart
Wells:
10-well
Price (USD)
178.00
Each
Add to cart
Similar to NuPAGE™ Bis-Tris gels, Bolt™ Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for Western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt Bis-Tris Plus gels to obtain optimal results for day-to-day protein separation needs.

Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES or MOPS SDS running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

• Optimized run conditions—separate proteins using constant voltage in approximately 20 minutes
• Better protein integrity—neutral pH sample preparation process preserves proteins
• Improved protein separation—offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than tris-glycine based gels
• Wedge-shaped wells—easier sample loading and up to two times higher sample volume than other 1.0mm-thick gels

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage10%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris Plus
Recommended ApplicationsDenaturing
Sample Loading Volumeup to 60 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life16 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineBolt™, WedgeWell™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit SizeEach

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What does it mean when bands appear to be getting narrower (or "funneling") as they progress down a protein gel?

There may be too much beta-mercaptoethanol (BME), sample buffer salts, or dithiothreitol (DTT) in your samples. If the proteins are over-reduced, they can be negatively charged and actually repel each other across the lanes causing the bands to get narrower as they progress down the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How much methanol is required per transfer when using the Mini Blot Module and Bolt Bis-Tris Plus gels?

We recommend using 1x Bolt Transfer buffer with 10% methanol.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all Bolt™ Bis-Tris Plus Mini Protein Gels, 10%, 1.0 mm, WedgeWell™ format FAQs