Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Invitrogen™

Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents

Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent.
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Catalog NumberProduct TypeQuantitation RangePackaging Type
P11495Quant-iT PicoGreen dsDNA Reagent50 pg to 2 μg10 x 100 μL Tubes
P7581Quant-iT PicoGreen dsDNA Reagent50 pg to 2 μg1 x 1 mL Bottle
P11496Quant-iT PicoGreen dsDNA Assay Kit50 pg to 2 μg10 x 100 μL Tubes
P7589Quant-iT PicoGreen dsDNA Assay Kit50 pg to 2 μg1 x 1 mL Bottle
Catalog number P11495
Price (USD)
690.00
Each
Add to cart
Product Type:
Quant-iT PicoGreen dsDNA Reagent
Quantitation Range:
50 pg to 2 μg
Packaging Type:
10 x 100 μL Tubes
Price (USD)
690.00
Each
Add to cart
Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers.
Rapidly detect as little as 0.25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products.

PicoGreen dsDNA quantitation reagents are
• Significantly more sensitive than UV absorbance readings, saving precious sample amounts
• Specific for dsDNA in the presence of RNA
• Easy to use—just add the dye working solution to the sample, incubate five minutes, and read
• Suitable for use in 96- and 384-well plate formats
• Compatible with most fluorescence-based microplate readers and fluorometers
• Picogreen aproximate fluorescence absorption/emission maxima is 502/523 nm, bound to dsDNA

Applications
The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
No. of Reactions200 Assays (2 mL assay volume)
Packaging Type10 x 100 μL Tubes
Quantitation Range50 pg to 2 μg
Product LinePICOGREEN, Quant-iT
Product TypeQuant-iT PicoGreen dsDNA Reagent
Shipping ConditionRoom Temperature
Unit SizeEach

Frequently asked questions (FAQs)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

View all Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents FAQs