Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size
Invitrogen™

Power Blotter Pre-cut Membranes and Filters, nitrocellulose, regular size

These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for transfer of proteins from two mini-size or one midi-sizeRead more
Have Questions?
Catalog NumberQuantity
PB7320
Catalog number PB7320
Price (USD)
364.00
Each
Add to cart
Price (USD)
364.00
Each
Add to cart
These Invitrogen Power Blotter Pre-cut Membranes and Filters are designed for transfer of proteins from two mini-size or one midi-size gel using the Power Blotter System. The pre-cut membranes and filters include 20 sheets of nitrocellulose and 80 sheets of absorbent blotting paper (0.85 mm thickness). Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.

Features of nitrocellulose membranes:
• High-quality—pure 100% nitrocellulose membranes with high surface area and excellent uniformity
• High-binding affinity—provides excellent protein binding (209 μg/cm2), blocks easily, and produces very low background in chemiluminescent and fluorescent western blotting
• Convenient—pre-cut nitrocellulose sheets are ready to use for transfer and blotting experiments from two standard precast or homemade mini gels or one midi gel (8 cm x 13 cm); no need to cut your own membranes, minimizing damage and contamination
• Validated—membrane and filter when combined provide the thickness and buffer capacity needed for optimal performance in the Power Blotter System

The nitrocellulose membrane (0.2 μM pore size) is composed of 100% pure nitrocellulose to provide high-quality transfers. Nitrocellulose is a popular binding matrix for western blotting because of its high affinity for proteins and compatibility with a variety of detection methods (e.g., chemiluminescence, chromogenic, fluorescence). The pre-cut membrane/filter paper sandwiches fit two mini-size or one midi-size gel.

Learn more about the Power Blotter System ›

Related products
Power Blotter Pre-cut Membranes and Filters, nitrocellulose, mini
Power Blotter Pre-cut Membranes and Filters, PVDF, mini
Power Blotter Pre-cut Membranes and Filters, PVDF, regular size

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Length (Metric)13 cm
Width (Metric)8.5 cm
FormatTransfer Stack
MaterialNitrocellulose
Pore Size0.2 μm
Shipping ConditionRoom Temperature
Dimensions (LxW)Regular, 13 cm x 8.5 cm
Thickness0.85 mm
Product LinePower Blotter
Sufficient For20 Blots
Unit SizeEach
Contents & Storage
20 regular-size transfer stacks (13 cm x 8.3 cm) composed of 20 sheets of nitrocellulose and 80 sheets of filter paper (0.85 mm thickness)

Frequently asked questions (FAQs)

How can I store, strip, and reuse my western blot?

For nitrocellulose or PVDF membrane following Western blot detection using a chemiluminescent or fluorescent substrate system: Following transfer, air dry the membrane and place in an envelope, preferably on top of a supported surface to keep the membrane flat. The blot can be stored indefinitely at -80 degrees C. When ready to reprobe, prewet the PVDF blot with alcohol for a few seconds, followed by a few rinses with pure water to reduce the alcohol concentration. Then proceed as normal with blocking step.

FOR STRIPPING/REPROBING OF MEMBRANES: Harsh protocol (see NOTE below for modifications)

1) Submerge the membrane in stripping buffer (100 mM BME, 2% SDS, 62.5 mM Tris-HCl, pH 6.7) and incubate at 50 degrees C for 30 min with occasional agitation. If more stringent conditions necessary, incubate at 70 degrees C.

2) Wash 2 x 10 min in TBS-T/PBS-T at room temperature.

3) Block the membrane by immersing in 5% blocking reagent TBS-T or PBS-T for 1 hr at room temperature.

4) Immunodetection

NOTE: Often you don't need such harsh conditions to remove antibodies from their proteins. The stringency of one or several of the variables can be decreased: lower the temperature, decrease the time, less BME, less SDS, etc. An especially mild but still often effective stripping protocol is lower pH incubation. Example: pH 2.0 Tris 50-100 mM, 30-60 min incubation (you may do two incubations if you wish). Then rinse and block as usual. If you do not wish to re-use the membrane immediately after stripping, you can store the membrane in plastic wrap (wet, you do not want it to dry out). Another simple, mild stripping buffer is 0.1 M glycine•HCl (pH 2.5-3.0), incubation 30 min to 2 hrs room temperature or 37 degrees C, depending on the antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I transfer EMSA gels using the Power Blotter System/Power Blotter XL System?

Yes. You may use the same method that was used with the G2 Fast Blotter: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/transfer-emsa-gels-using-g2-fast-blotter.html

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.