Quant-it™ RiboGreen RNA Assay Kit and RiboGreen RNA Reagent, RediPlate™ 96 RiboGreen™ RNA Quantitation Kit
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Invitrogen™

Quant-it™ RiboGreen RNA Assay Kit and RiboGreen RNA Reagent, RediPlate™ 96 RiboGreen™ RNA Quantitation Kit

Rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assays are enabled with the RediPlate 96 RiboGreen RNA Quantitation Kit. The microplate is pre-loaded with RiboGreen Reagent, which produces bright green fluorescence upon binding to RNA or DNA.
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Catalog NumberProduct TypeQuantitation Range
R32700RediPlate 96 RiboGreen RNA Kit3 to 200 ng
R11490Quant-iT RiboGreen RNA Kit1 to 200 ng
R11491Quant-iT RiboGreen RNA Reagent1 to 200 ng
Catalog number R32700
Price (USD)
308.00
Each
Add to cart
Product Type:
RediPlate 96 RiboGreen RNA Kit
Quantitation Range:
3 to 200 ng
Price (USD)
308.00
Each
Add to cart
Quantify as little as 1 ng of RNA with the Quant-iT RiboGreen RNA Assay Kit or the RediPlate 96 RiboGreen RNA Quantitation Kit. These RNA quantification kits contain Quant-iT RiboGreen RNA reagent, which is one of the most sensitive detection dyes for the quantitation of RNA in solution, with linear fluorescence detection in the range of 1–200 ng of RNA.
Quant-iT RiboGreen RNA Assay Kit
Containing one of the most sensitive detection dyes for the quantitation of RNA, the Quant-iT RiboGreen RNA Assay Kit and the RediPlate 96 RiboGreen RNA Quantitation Kit offer linear fluorescence detection in the range of 1–200 ng of RNA. Both assay kits contain Quant-iT RiboGreen RNA reagent as well as buffers and RNA standards.

RediPlate 96 RiboGreen RNA Quantitation Kit
The RediPlate 96 RiboGreen RNA Quantitation Kit is a rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assay. The microplate is pre-loaded with RiboGreen reagent, which shows bright green fluorescence upon binding to RNA or DNA. Just add buffer and samples to the microplate wells, wait 10 minutes, and read the fluorescence in any standard fluorescence-based microplate reader. The versatile RediPlate 96 design is ideal for either high-throughput RNA quantitation or the measurement of a small sample numbers.

The RediPlate 96 RiboGreen RNA Quantitation Kit also offers the following benefits:
Automatable—a 10 minute incubation time with no separation steps makes the assay ideal for automated, high-throughput measurements
Precise—the average coefficient of variation for replicate assays is ≤5% using a robotic liquid handling system
Convenient—each plate includes pre-measured RNA standards and is ready for immediate use
Easy to read—the assay can be performed using standard fluorescence-based microplate readers

Applications
The RediPlate 96 RiboGreen RNA Quantitation Kit is ideal for measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR, northern blot analysis, S1 nuclease assays, RNase protection assays, and cDNA library preparations.

Quant-iT RiboGreen RNA Reagent
The Quant-iT RiboGreen RNA Reagent is one of the most sensitive fluorescent dyes for the quantitation of RNA in solution, with linear fluorescence detection in the range of 1-200 ng of RNA. This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA standard.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
Quantitation Range3 to 200 ng
Product LineRIBOGREEN, RediPlate
Product TypeRediPlate 96 RiboGreen RNA Kit
Shipping ConditionRoom Temperature
Unit SizeEach

Frequently asked questions (FAQs)

When I try to run RediPlate 96 RiboGreen RNA Kit (Cat. No. R32700) on my Varioskan LUX instrument, it says there is no plate. What should I do?

When using a strip plate with the Varioskan LUX plate reader, ensure that there is a strip covering the D6 well, as this is where the instrument checks for the presence of a plate. The strip does not need to be active; it just needs to be in place for the instrument to verify the plate's presence. Alternatively, you can resolve this issue by adjusting the software settings. Go to the general settings and uncheck the box labeled "Check plate and tip prime vessel before session execution." This will immediately solve the problem.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does the Quant-it RiboGreen RNA Assay Kit and RiboGreen RNA Reagent (Cat. No. R32700) work with double-stranded RNA (dsRNA)?

We have not validated measurement of dsRNA with the Quant-it RiboGreen RNA Assay Kit and RiboGreen RNA Reagent (Cat. No. R32700). If you are using RiboGreen dye to measure dsRNA, then you should use your own dsRNA standards, rather than the standards provided with the kit, to get an accurate measurement.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center