StemPro™ Human Adipose-Derived Stem Cell Kit
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Gibco™

StemPro™ Human Adipose-Derived Stem Cell Kit

Human adipose-derived stem cells (ADSCs) are isolated from human lipoaspirate tissue and cryopreserved from primary cultures. ADSCs have phenotypic andRead more
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Catalog number R7788110
Price (USD)
1,032.00
Each
Add to cart
Price (USD)
1,032.00
Each
Add to cart
Human adipose-derived stem cells (ADSCs) are isolated from human lipoaspirate tissue and cryopreserved from primary cultures. ADSCs have phenotypic and functional characteristics very similar to those of bone marrow-derived mesenchymal stem cells. Before cryopreservation, the ADSCs are expanded for one passage in MesenPRO RS™ Medium, a reduced-serum (2%) medium that improves MSC expansion compared to conventional serum supplemented media. The StemPro™ Human Adipose-Derived Stem Cell Kit contains cryopreserved normal human ADSCs and MesenPRO RS™ Medium - together, these components ensure optimal ADSC growth and expansion. Each lot of ADSCs originates from a single donor of human lipoaspirate tissue. Each vial of ADSCs contains cells that can differentiate into multiple mature cell phenotypes in vitro, including adipocytes, osteoblasts, and chondrocytes. ADSCs can be expanded to 4-5 passages before they lose their ability to grow or differentiate into all potential phenotypes. The ADSCs express the following flow cytometry cell-surface protein profile: positive CD29, CD44, CD73, CD90, CD105, CD166; negative CD14, CD31, CD45, Lin1.

Applications: Formulated for the growth and expansion of mesenchymal stem cells.

Note: MesenPRO RS™ Medium (Cat. no. 12746-012), composed of MesenPRO RS™ Growth Supplement and MesenPRO RS™ Basal Medium, is available for purchase separately.
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal Law restricts this device to sale by or on the order of a physician.
Specifications
Cell TypeStem Cell (Mesenchymal)
SpeciesHuman
TissueLipoaspirate
Product LineGibco™, StemPro™
Quantity1 kit
Unit SizeEach
Contents & Storage
This kit is shipped in two parts with separate storage requirements. These components are not sold individually:

–StemPro™ Human Adipose-Derived Stem Cells (one vial containing ∼1 x 106 cells; store in liquid nitrogen) and MesenPRO RS™ Growth Supplement (10 ml; store in the dark at -5 to -20°C)
–MesenPRO RS™ Basal Medium (500 ml; store in the dark at 2-8°C)

Frequently asked questions (FAQs)

What kinds of growth factors are used in mesenchymal stem cell (MSC) research?

Growth factors used for MSC maintenance and self-renewal: LIF, FGF-basic, EGF, HGF, PDGF, and Wnt3.

Growth factors or cytokines for MSC differentiation: BMP-2and TGF-β1.

(Arthritis Res Ther 9:204 (2007)

View our Gibco™ MSC research products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I culture mesenchymal stem cells (MSCs) in vitro?

MSCs may be cultured in either serum-containing medium or serum-free medium. The standard culturing conditions are DMEM, low glucose with 10% FBS. MSCs can also be grown in reduced-serum (2%) Gibco MesenPRO RS Medium (Cat. No. 12746-012), or in serum-free Gibco StemPro MSC SFM XenoFree Medium (Cat. No. A10675) and Gibco StemPro MSC SFM (Cat. No. A10332). In general, MSCs grow better under hypoxic conditions (2% O2).

How do you characterize human mesenchymal stem cells (MSCs)?

Human MSCs can be identified by flow cytometry, typically displaying CD73+, CD90+, CD105+, CD11b-, and CD45- marker characteristics (Blood 109:4245 (2007)). Human MSC expresses Oct4, Sox2, and Rex-1; these may be verified using RT-PCR (Arthritis Res Ther 9:204 (2007)).

What are mesenchymal stem cells?

Mesenchymal stem cells (MSCs) are multipotent cells isolated primarily from bone marrow or fat tissues that exhibit the ability to differentiate into bone, cartilage, and fat cells. Under normal cell culture conditions, MSCs isolated from bone marrow are spindle shaped with the unique ability to adhere to uncoated plastic culture dishes (Arthritis Res Ther 9:204 (2007)).