SlowFade™ Gold Antifade Mountant with DAPI
SlowFade™ Gold Antifade Mountant with DAPI
Invitrogen™

SlowFade™ Gold Antifade Mountant with DAPI

Green features
SlowFade Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides.Read more
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Catalog NumberQuantity
S3693810 mL
S369395 x 2 mL
S369422 mL
Catalog number S36938
Price (USD)
255.00
Each
Add to cart
Quantity:
10 mL
Price (USD)
255.00
Each
Add to cart

SlowFade Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. SlowFade Gold Antifade Mountant is glycerol-based, with no mixing or curing required, which makes it ideal for immediate viewing of the sample. It comes ready-to-use—just apply a drop to the sample, add a coverslip, and image. It is available with or without DAPI nuclear stain.

Key attributes:
• Protects dyes from fading during imaging
• Ready-to-use liquid, ideal for immediate sample viewing
• Mounted samples are stable for weeks
• Maintains signal strength—little to no quenching
• Ideal for Alexa Fluor dyes

Select the antifade mounting or optical clearing reagent that matches your fluorescence imaging needs ›

SlowFade Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP. For superior antifade protection of fluorescent proteins and fluorescent dyes in cultured cells or thin tissue slices, SlowFade Diamond Antifade Mountant is recommended. For high resolution or to image thicker tissue or 3D cell culture with a focal depth of 0–500 μm, try SlowFade Glass Antifade Mountant.

Select best SlowFade antifade reagent for your experimental needs ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Microscope
Product LineSlowFade™
Product TypeMounting Media
Quantity10 mL
Solution TypeAnti-fade Mountant
Green FeaturesLess hazardous, sustainable packaging
Label or DyeDAPI
Shipping ConditionRoom Temperature
FormLiquid
Unit SizeEach
Contents & Storage
Storage at room temperature is recommended but can also be stored frozen (-5° to -30°C). Protect from light.

Frequently asked questions (FAQs)

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does Slowfade Antifade Mountant require using a nail polish for sealing?

No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.