SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in DMSO
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SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in DMSO
Invitrogen™

SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in DMSO

SYBR™ Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA)Read more
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Catalog NumberQuantity
S7563500 μL
S758520 x 50 μL
S75671 mL
Catalog number S7563
Price (USD)
461.00
Each
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Quantity:
500 μL
Request bulk or custom format
Price (USD)
461.00
Each
Add to cart
SYBR™ Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. Because SYBR™ Green I has greater sensitivity for dsDNA, it is especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. With exceptionally low background fluorescence and spectral characteristics that closely match light sources and filter sets in existing instruments, SYBR™ Green I stain is ideal for use with laser scanners. SYBR™ Green I stain provides:

• Sensitivity—at least four times greater than ethidium bromide for DNA in agarose gels
• Ease of use—gels soaked in diluted stain can be visualized without desalting
• Compatibility—with UV transilluminators, gel documentation systems, and laser scanners
• Flexibility—for use with a broad range of applications, including DNA typing, PCR-based assays, DNA damage assays, analysis of complex samples, and real-time PCR detection

Product use
One mL stains 100 minigels. Various pack sizes are available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodFluorescence
Label or DyeSYBR Green I
Product TypeNucleic Acid Gel Stain
Quantity500 μL
Shipping ConditionRoom Temperature
Target MoleculeDNA
Unit SizeEach
Contents & Storage
• Supplied as a 10,000X concentrate in DMSO

Store at -20°C, protected from light in a dessicator.

Frequently asked questions (FAQs)

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the recommended filter for my gel documentation system?

Please go here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html) and click on the “Filter Recommendations” tab to see filter recommendations for use with SYBR Safe DNA Gel Stain. Note that the excitation and emission spectra of SYBR Safe DNA Gel Stain are very similar to those of SYBR Green I, SYBR Green II, and SYBR Gold dyes, as well as fluorescein (FITC). Therefore, filters appropriate for these dyes can also be used. A camera filter is not required with the Safe Imager Blue-Light Transilluminator; the amber filter provided with the instrument serves this purpose.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I use the ethidium bromide filters on my camera to image SYBR dyes?

This is not recommended. Most deep amber/orange ethidium bromide filters have a cutoff value around 550 nm. The SYBR Green dyes emit at 520 nm, which will not be detected using this filter.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Is SYBR Safe DNA Gel Stain the same as SYBR Green I dye?

All SYBR dyes have similar spectral properties, but have different chemical compositions. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain (Cat. No. S33102) was specifically developed as a safer alternative to ethidium bromide. SYBR Green I (Cat. No. S7585) is an ultrasensitive stain for dsDNA, and SYBR Green II (Cat. No. S7564) is a highly sensitive stain for RNA and ssDNA. All SYBR dyes are optimally excited by the Safe Imager Blue-Light Transilluminator.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

How should I dispose of SYBR DNA Gel Stain?

Disposal regulations vary. Please contact your safety office or local municipality for disposal guidelines.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

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