iBind™ Western Starter Kit
iBind™ Western Starter Kit
Invitrogen™

iBind™ Western Starter Kit

The iBind Western Starter Kit includes an iBind Western Device as well as all of the consumables and reagents necessaryRead more
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Catalog number SLF1000S
Price (USD)
1,622.00
Each
Add to cart
Price (USD)
1,622.00
Each
Add to cart
The iBind Western Starter Kit includes an iBind Western Device as well as all of the consumables and reagents necessary to get started using this automated western-processing device. The iBind Western Device performs every step in the western protocol from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source, shakers, or trays.

Learn more about the iBind western systems ›

The iBind Western Device works exclusively with iBind Cards and the iBind Solution Kit to process one mini-membrane for immunodetection. The iBind device provides automated convenience and superior reproducibility over traditional manual methods. Each solution is released from the iBind device wells to an iBind Card via SLF in ∼2.5 hours. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions over the membrane, increasing the antigen-antibody interaction.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Capacity1 mini blot (8 x 8 cm)
Run Time∼2.5 hours
Detection MethodChromogenic, Chemiluminescence, Fluorescence
Membrane CompatibilityNitrocellulose, PVDF
Product LineiBind™
Product TypeStarter Kit
Quantity1 kit
Unit SizeEach
Contents & Storage
• 1 iBind™ Western Device
• 10 iBind™ Cards (store at room temperature)
• 1 iBind™ Solution Kit (store at 2–8°C)

Frequently asked questions (FAQs)

Do you offer additional years of service for the iBind Western Device?

No, we do not provide service contracts or extended warranties for these devices. The warranty is limited to 1 year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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