iBind™ Solution Kit
iBind™ Solution Kit
Invitrogen™

iBind™ Solution Kit

The iBind Solutions Kit is intended for use with the iBind Western Device, which is an automated western-processing device thatRead more
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Catalog NumberType
SLF1020Solution Kit
SLF1020X4Solution Kit
Catalog number SLF1020
Price (USD)
96.00
Each
Add to cart
Type:
Solution Kit
Price (USD)
96.00
Each
Add to cart
The iBind Solutions Kit is intended for use with the iBind Western Device, which is an automated western-processing device that performs every step in the western workflow from blocking to washes to antibody incubations. The iBind solutions act as the blocking, washing, and dilution solutions in the iBind immunodetection process and have been optimized for ideal viscosity. Each solution is released from the iBind device wells to the iBind Card through sequential lateral flow (SLF); the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

Learn more about the iBind western systems ›

The iBind Solutions Kit, together with the iBind Western Device and iBind Cards, bring automation and simplicity to the western protocol. Each iBind Solutions Kit contains sufficient solution for 10 mini-membrane reactions. An iBind Western Device is required for use of this kit. For fluorescent applications use the iBind Fluorescent Detection (FD) Solution Kit.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodColorimetric, Chromogenic, Chemiluminescence
For Use With (Application)Western Blot
For Use With (Equipment)iBind Western Device
Membrane CompatibilityNitrocellulose, PVDF
Product LineiBind
TypeSolution Kit
No. of Reactions10 mini membrane reactions
Sample TypeProtein
Unit SizeEach
Contents & Storage
Store at 2–4°C.

Frequently asked questions (FAQs)

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What iBind Solution kits are available?

We offer the iBind Solution kit and the iBind Fluorescent Detection (FD) Solution kit. For standard chemiluminescent or chromogenic detection, we recommend the iBind Solution kit (Cat. No. SLF1020) and for fluorescent detection, we recommend the iBind Fluorescent Detection (FD) Solution kit (Cat. No. SLF1019).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all iBind™ Solution Kit FAQs