iBind™ Flex Western Starter Kit
iBind™ Flex Western Starter Kit
Invitrogen™

iBind™ Flex Western Starter Kit

The iBind Western Starter Kit includes one iBind Flex Western Device, one box of iBind Flex Cards, and one iBindRead more
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Catalog number SLF2000S
Price (USD)
3,315.00
Each
Add to cart
Price (USD)
3,315.00
Each
Add to cart
The iBind Western Starter Kit includes one iBind Flex Western Device, one box of iBind Flex Cards, and one iBind Flex Solution Kit.

The iBind Flex Western Device is an automated western-processing device that performs every step from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source, shakers, or trays. Each solution is released from the iBind Flex device wells to an iBind Flex Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction.

Learn more about the iBind western systems ›

The iBind Flex western detection procedure minimizes hands-on time and is highly reproducible. Setup time is fast: within 2.5 h you are ready for the detection step. Compared to manual methods, the iBind Flex western system requires less primary antibody, enables reproducible results, and is compatible with downstream detection protocols.

The iBind Flex Western Device includes three user-interchangeable well inserts to process up to one midi blot, two mini blots, or six vertically-cut strip blots when used with the iBind Flex Cards and iBind Flex Solution Kit or iBind Flex Fluorescent Detection (FD) Solution Kit.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Capacityone midi blot, six vertically-cut strip blots, two mini blots
Kit Contents10 iBind cards, 1 iBind solution kit, Western device
Run Time∼2.5 hours
Shipping ConditionRoom Temperature
Detection MethodChromogenic, Chemiluminescence, Fluorescence
Membrane CompatibilityNitrocellulose, PVDF
Product LineiBind™
Product TypeStarter Kit
Quantity1 kit
Unit SizeEach
Contents & Storage
• 1 iBind Flex Western Device
• 10 iBind Flex Cards, store at room temperature.
• 1 iBind Flex Solution Kit, store at 2–8°C.

Frequently asked questions (FAQs)

Can I use conjugated primary antibodies with the iBind Flex Western Device?

Yes. When using a conjugated primary antibody and no secondary antibody, add the diluted primary antibody to well 1 and replace diluted secondary antibody (well 3) with 1X iBind Flex/iBind Flex FD Solution. Alternatively, for faster time to results, add the diluted primary antibody to well 1, leave wells 2 and 3 empty, and add 1X iBind Flex/ iBind Flex FD Solution to well 4. This procedure will save time (~60 min) but may result in higher background.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What procedure should I follow when stripping and reprobing my blot processed using the iBind Western Device or iBind Flex Western Device?

To strip and reprobe your blot processed using the iBind Western Device or iBind Flex Western Device, first strip the blot outside the system using your preferred protocol. After the stripping protocol, wash the blot in distilled water for 5 min. Reblocking is unnecessary as the iBind Solution will perform this step during reprobing. Finally, reprobe with new antibodies using the iBind Western Device or iBind Flex Western Device, starting with the membrane preparation step.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I multiplex antibodies using the iBind Western Device or iBind Flex Western Device?

Yes, for each midi blot, mini blot, or vertically cut strip, prepare appropriate primary antibodies together in a single tube of iBind FD/ iBind Flex FD Solution, Prepare appropriate secondary antibodies together in a different tube of iBind FD/ iBind Flex FD Solution. Do not mix primary and secondary antibodies prior to loading in the device. All primary and secondary antibodies should be diluted according to the recommendations outlined in the user manual or quick reference card. When detecting several targets of the same species, the same concentration of secondary antibody can be used as when detecting one target. There is no need to increase the concentration for each additional target. Load multiplexed antibodies into the device as normal.
Caution: Consider cross-reactivity of secondary antibodies when multiplexing (e.g., rabbit anti-goat IgG and goat anti-mouse IgG are likely to cross-react).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I use the iBind Western Device or iBind Flex Western Device with Alexa Fluor Plus conjugated secondary antibodies?

Yes, both the iBind Western Device and iBind Flex Western Device work well with Alexa Fluor Plus conjugated secondary antibodies.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

With the iBind Western Device and iBind Flex Western Device, how do I know if I am rolling the membrane firmly enough?

When firmly rolling, the iBind/iBind Flex Card will slightly dip in the membrane area, causing the top and bottom of the card to lift. See the iBind/iBind Flex user manual for additional details.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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