GeneRuler High Range DNA Ladder
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GeneRuler High Range DNA Ladder
Thermo Scientific™

GeneRuler High Range DNA Ladder

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Thermo Scientific GeneRuler High Range DNA Ladder is recommended for fast sizing and approximate quantification of high molecular weight double-strandedRead more
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Catalog NumberQuantity
SM13525 x 50 μg
SM135150 μg
Catalog number SM1352
Price (USD)
504.00
Each
Add to cart
Quantity:
5 x 50 μg
Request bulk or custom format
Price (USD)
504.00
Each
Add to cart
Thermo Scientific GeneRuler High Range DNA Ladder is recommended for fast sizing and approximate quantification of high molecular weight double-stranded DNA on agarose gels (in 1.5 hour, 0.4% agarose gel). The ladder is a mixture of chromatography-purified individual DNA fragments.

GeneRuler High Range DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase (see Protocol in the Resources.)

The ladder is supplied with 6X Orange DNA Loading Dye for sample DNA.

Highlights

• Ideal for both DNA sizing and approximate quantification
• Ideal for fast sizing of high molecular weight DNA fragments
• Sharp bands
• Supplied with loading dye for sample DNA
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration6X
Gel TypeAgarose
Label or DyeXylene Cyanol FF, Orange G
Product TypeHigh Range DNA Ladder
Ready to LoadNo
Size Range10171 to 48502 bp
Gel CompatibilityAgarose Gels
Green FeaturesSustainable packaging
Product LineGeneRuler
Quantity5 x 50 μg
Unit SizeEach

Frequently asked questions (FAQs)

Are the Thermo Scientific DNA ladders compatible with GelRed and GelGreen gel stains?

The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes.

Which Thermo Scientific DNA ladders are optimal for fast electrophoresis separation?

For fast sizing and quantification of double-stranded DNA, we recommend using FastRuler DNA Ladders. These ladders are easily resolved in a short separation distance after an 8-14 min run.

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).

What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.