RiboRuler Low Range RNA Ladder, ready-to-use
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RiboRuler Low Range RNA Ladder, ready-to-use
Thermo Scientific™

RiboRuler Low Range RNA Ladder, ready-to-use

Thermo Scientific RiboRuler Low Range RNA Ladder 100-1000 bp, ready-to-use, is a mixture of 7 chromatography purified single-stranded RNA transcriptsRead more
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Catalog number SM1833
Price (USD)
215.00
Each
Add to cart
Request bulk or custom format
Price (USD)
215.00
Each
Add to cart

Thermo Scientific RiboRuler Low Range RNA Ladder 100-1000 bp, ready-to-use, is a mixture of 7 chromatography purified single-stranded RNA transcripts (100–1000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.

This ladder is premixed with loading dye.

RiboRuler Low Range RNA Ladder, ready-to-use, is composed of chromatography-purified transcripts what makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically. To minimize the possibility of contamination during the use the ladder is aliquotted in smaller volumes.

RiboRuler Low Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase (see protocol in the Resources) and is ideal for Northern blots.

Highlights

  • Sharp bands of uniform intensity
  • Easy-to-remember band sizes and quantities
  • Approximate RNA quantification by predetermined concentration of ladder bands
  • Supplied with 2X RNA Loading Dye

Applications

  • RNA sizing and quantification on native or denaturing gels
  • Northern blotting.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration2X
Gel TypeAgarose
Label or DyeXylene Cyanol FF, Bromophenol Blue, Ethidium Bromide
Product TypeDNA Ladder
Size Range100 to 1000 bp
Product LineRiboRuler
Quantity5 x 40 μL
Unit SizeEach

Frequently asked questions (FAQs)

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).

What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.

Can I use ready-to-use RiboRuler RNA Ladders in native gel electrophoresis?

Ready-to-use RiboRuler RNA Ladders are not suitable for native gel electrophoresis since they are in a loading buffer that contains 47.5% formamide and 0.0125% SDS.

What ladder do you recommend for running RNA on Invitrogen E-Gel EX Agarose Gels?

Depending on the RNA fragment size, we recommend the following ladders:

  • RiboRuler Low Range RNA ladder, (100 bp-1,000 bp) (Cat. No. SM1833)

  • RiboRuler High Range RNA ladder, (200 bp-6,000 bp) (Cat. No. SM1823)


Find additional tips, troubleshooting help, and resources within our Nucleic Acid Gel Electrophoresis and Blotting Support Center.