Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)
Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)
Invitrogen™

Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)

Disufide crosslinks of cystines in proteins can be reduced to cysteine residues by TCEP (tris-(2-carboxyethyl)phosphine). Unlike DTT (dithiothreitol), TCEP doesRead more
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Catalog number T2556
Price (USD)
157.00
Each
Add to cart
Price (USD)
157.00
Each
Add to cart
Disufide crosslinks of cystines in proteins can be reduced to cysteine residues by TCEP (tris-(2-carboxyethyl)phosphine). Unlike DTT (dithiothreitol), TCEP does not contain thiols and therefore usually does not need to be removed prior to thiol modification.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Shipping ConditionRoom Temperature
FormSolid
Quantity1 g
Unit SizeEach
Contents & Storage
Store at room temperature.

Frequently asked questions (FAQs)

Can other reducing agents other than DTT or BME be used to reduce proteins prior to electrophoresis? For example, what about TCEP (Tris Carboxy Ethyl Phosphene)?

TCEP, Tris Carboxy Ethyl Phosphene is an alternative sulfhydryl reducing agent for protein samples. It is an extremely potent and effective reducing agent for particularly ‘difficult' proteins. It is compatible with the Tris-Glycine gels and NuPAGE gels. It should be added to the sample buffer for these systems. 20 mM final (maximum) concentration is sufficient for samples. You may add alkylating agents, e.g. Iodine (50 mM Iodoacetic acid), to prevent re-forming of S-S bonds but it is not necessary. Do not heat because this will hydrolyze much of your sample. Instead let the sample sit for several minutes at RT and then load.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.