WesternBreeze™ Chemiluminescent Kit, anti-mouse
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Invitrogen™

WesternBreeze™ Chemiluminescent Kit, anti-mouse

WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directlyRead more
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Catalog number WB7104
Price (USD)
600.00
Each
Add to cart
Price (USD)
600.00
Each
Add to cart
WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use CDP-Star® chemiluminescent substrate for alkaline phosphatase. Protein bands can be captured either by X-ray film or CDP-Star® compatible imaging system. WesternBreeze® Chemiluminescent Kit offers:

• High specificity, clean background
• Ultra-sensitivity-femtogram levels detectable
• Long-lasting signals-up to 5 days
• Results in less than 3 hours
• Permanent photographic image
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ReactivityMouse
Shipping ConditionWet Ice
Substrate TypeAP (Alkaline Phosphatase) Substrate
Detection MethodChemiluminescence
Membrane CompatibilityNitrocellulose, PVDF
Product LineWesternBreeze™
Product TypeWestern Blot Kit
Cross ReactivityMouse
Quantity1 kit
Unit SizeEach
Contents & Storage
The WesternBreeze™ Chemiluminescent Kits include blocking solutions, primary antibody diluent, ready-to-use secondary antibody solution (anti-mouse, anti-rabbit or anti-goat), ready-to-use chemiluminescent substrate, wash solutions, incubation trays, pre-cut filter papers, polyester sheet for even substrate development on the membrane. Each kit contains complete reagents for 20 blots. Store the kits at +4°C. Guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.