Novex™ Tris-Glycine Plus Midi Protein Gels, 8 to 16% ,1.0 mm
Novex™ Tris-Glycine Plus Midi Protein Gels, 8 to 16% ,1.0 mm
Invitrogen™

Novex™ Tris-Glycine Plus Midi Protein Gels, 8 to 16% ,1.0 mm

Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers.
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Catalog NumberWells
WXP81612BOX12 + 2-well
WXP81612BOXA12 + 2-well, w/adapters
WXP81620BOX20-well
WXP81620BOXA20-well, w/adapters
WXP81626BOX26-well
WXP81626BOXA26-well, w/adapters
Catalog number WXP81612BOX
Price (USD)
210.00
Each
Add to cart
Wells:
12 + 2-well
Price (USD)
210.00
Each
Add to cart
Novex Tris-Glycine Plus Midi Protein gels are improved polyacrylamide gels based on traditional Laemmli protein electrophoresis technology, allowing the use of Laemmli sample and running buffers. The gels offer reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Plus Midi gels are a 1.0-mm thick, wider format (8 cm x 13 cm) gel for higher throughput electrophoresis of protein samples.

Features of Novex Tris-Glycine Plus gels include:
Improved shelf life—store gels for up to 12 months at 4°C
Flexibility—use for native and denaturing protein assays
Fast run conditions—quickly separate your proteins using constant voltage in less than 60 minutes

Choose the right gel format for your experiments
Novex Tris-Glycine Plus Midi gels come in fixed concentrations of 10% and 12%, as well as gradient concentrations of 4–12%, 4–20%, and 8–16%. Select from our many well formats, including 12+2-, 20-, and 26-well.

Run your proteins in native or denatured form
Novex Tris-Glycine Plus gels do not contain SDS and so can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC26754). For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer (LC2673) and Novex Tris-Glycine Native Running Buffer (LC2672). The gels can be run using our XCell4 SureLock Midi-Cell (WR0100) or conveniently with the Bio-Rad Criterion Cell using our adapters (WA0999).

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer (LC3675) when performing traditional wet transfer. Rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
AdaptersNo Adapters
For Use With (Equipment)XCell4 SureLock Midi-Cell, SureLock Tandem Midi Gel Tank
Gel Percentage8 to 16%
Gel SizeMidi
Gel Thickness1.0 mm
Gel TypeTris-Glycine Plus
Sample Loading VolumeUp to 45 μL + 15 μL
Separation Range6 to 250 kDa
Separation TypeDenaturing, Native
Shelf Life310 Days
Wells12 + 2-well
Mode of SeparationMolecular Weight
Product LineNovex™
Quantity10 Gels/Box
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)13 cm
Unit SizeEach

Frequently asked questions (FAQs)

Can Tris-Glycine Plus Midi Gels be stored at room temperature?

No. They must be stored at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Do Tris-Glycine Plus Midi Gels require any specific buffers?

Tris-Glycine Plus Midi Gels can use standard Tris-Glycine sample and running buffers. If you want to run under native conditions, we recommend using sample and running buffers that do not contain SDS (or our Native Tris-Glycine premade buffers).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What in the chemistry changed to allow for a longer shelf life for Tris-Glycine Plus Midi Gels?

We were able to optimize shelf life of the gels through a proprietary gel formulation change. Unfortunately, we are unable to provide specific details on the chemical changes.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life of Tris-Glycine Plus Midi Gels?

Tris-Glycine Plus Midi Gels have a shelf life of up to 1 year depending upon gel percentages. The minimum shelf life of these gels is at least 6 months from date of manufacture.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can Tris-Glycine Plus Midi Gels be run in the Bio-Rad Criterion gel running tank?

Yes, Tris-Glycine Plus Midi Gels can be run in the Bio-Rad Criterion tank with the help of adapters. If you are interested in running these gels in the Bio-Rad Criterion tank, we recommend buying the versions of these gels that come with adapters.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.