Zip Alexa Fluor™ Rapid Antibody Labeling Kits
Zip Alexa Fluor™ Rapid Antibody Labeling Kits
Invitrogen™

Zip Alexa Fluor™ Rapid Antibody Labeling Kits

Invitrogen Zip Alexa Fluor Rapid Antibody Labeling kits allow you to efficiently and quickly label your precious antibody with Alexa Flour dyes to create an antibody conjugate that is ready to use within 15 minutes with 100% recovery.
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Catalog NumberQuantityExcitation/EmissionLabel or Dye
Z112333 Reactions490/525 nmAlexa Fluor 488
Z112343 Reactions555/580 nmAlexa Fluor 555
Z112353 Reactions650/665 nmAlexa Fluor 647
Catalog number Z11233
Price (USD)
584.00
Each
Add to cart
Quantity:
3 Reactions
Excitation/Emission:
490/525 nm
Label or Dye:
Alexa Fluor 488
Price (USD)
584.00
Each
Add to cart
Invitrogen Zip Alexa Fluor Rapid Antibody Labeling kits allow you to efficiently and quickly label your precious antibody with Alexa Flour dyes to create an antibody conjugate that is ready to use within 15 minutes with 100% recovery. Each kit contains everything needed for three separate labeling reactions of 100 μg of antibody. Simply add 1 mL water to component A containing Zip buffer, add 100 μg of antibody, incubate for 15 minutes, and your antibody is ready to use. Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry (IHC), primary detection, ELISAs, immunocytochemistry (ICC), and indirect FISH.

Create ready-to-use antibody conjugate in just 15 minutes with our Invitrogen Zip Alexa Fluor Rapid Antibody Labeling kits. These kits enable you to efficiently label your precious antibody with various Alexa Flour dyes.

Important features of Zip Alexa Fluor Rapid Antibody Labeling kits:
· Speed—antibody conjugates ready to use in 15 min
· Efficiency—100% antibody recovery
· Versatility—kits are useful for a wide range of applications
· Easy-to-use—no chemistry or conjugation experience needed
· Flexibility—choose from Alexa 488, 555, and 647

Each kit contains everything you need to perform three separate labeling reactions of 100 μg of antibody. Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry (IHC), primary detection, ELISA, immunocytochemistry (ICC), and indirect FISH.

Benefits of using Zip Alexa Fluor Rapid Antibody Labeling kits:

Specificity and versatility
A primary antibody that is directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Multiple primary antibodies of the same isotype or derived from the same species can be used in the same experiment if they are directly labeled with compatible fluorophores.

Robustness
Compared to traditional dyes, Alexa Fluor dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. When using Alexa Fluor dyes, higher degrees of labeling can usually be achieved without intramolecular quenching.

Customization is possible
Can’t find what you’re looking for in our stocked list? We can prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Kit ContentsReactive fluorescent label (3 vials), sodium bicarbonate (3 vials)
Product TypeLabeling Kit
Quantity3 Reactions
Excitation/Emission490/525 nm
Labeling Scale100 μg
Product LineAlexa Fluor™
Chemical ReactivityAmine
Label or DyeAlexa Fluor 488
ColorGreen
Labeling TargetIgG
Label TypeAlexa Fluor
Unit SizeEach
Contents & Storage
Store desiccated and protected from light in the freezer (-5°C to +30°C).

Frequently asked questions (FAQs)

With the Zip Alexa Fluor Rapid Antibody Labeling kits, can I store the labeled antibody after the 15 mins reaction?

No, it is intended for immediate use. If you wish to store your labeled antibody after the reaction, it should undergo a post-reaction clean-up using either size exclusion resin (such as Bio-Rad BioGel P-30) or Dye Removal Columns (Cat No. 22858).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do the Zip Alexa Fluor Rapid Antibody Labeling kits not require a post-reaction purification step?

This is because we have optimized the amount of dye to label 100 µg of antibody per reaction and after the incubation time of 15 mins, most of the reactive dye is either bound or unreactive due to spontaneous hydrolysis, leaving little or no reactive dye to label your sample.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am planning to use one of the Zip Alexa Fluor Rapid Antibody Labeling kits. My antibody is shipped in Tris buffer with BSA added as a stabilizer. Do I need to remove the BSA or Tris prior to the reaction?

Yes, the BSA and any component with a primary amine (such as the Tris buffer) would react with the amine-reactive Alexa Fluor dyes used in these labeling kits, resulting in less optimal labeling of the antibody. For small volume purification of the antibody, you may use either Nab Protein A/G Spin Kit, 0.2 mL (Cat No. 89950), Protein A/G Magnetic Agarose Beads (Cat No. 78609), or Melon Gel IgG Spin Purification Kit (Cat No. 45206).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For the Zip Alexa Fluor Rapid Antibody Labeling kits, why does the antibody have to be at a 1 mg/mL concentration?

The 1 mg/mL concentration of the antibody (100 µg of antibody in a 100 µL volume) allows for optimal reaction kinetics. A more dilute solution of antibody would exhibit suboptimal labeling due to a greater amount of reactive dye lost to spontaneous hydrolysis; the reactive group on the dyes is labile in aqueous solutions.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is included in the Zip Alexa Fluor Rapid Antibody Labeling kits?

Each kit contains 3 vials of solid sodium bicarbonate (Zip buffer reagent, Component A), 3 vials of the Alexa Fluor reactive dye (Component B), and a Quick Reference Card. Sufficient reagents are provided to label 100 µg of antibody per reaction (100 µL of a 1 mg/mL antibody solution), 3 reactions per kit.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.