FluoroBrite™ DMEM
FluoroBrite™ DMEM
Gibco™

FluoroBrite™ DMEM

Gibco™ FluoroBrite™ DMEM features a background fluorescence that is comparable to PBS and 90% lower than that emitted by standardRead more
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Catalog NumberQuantity
A189670210 x 500 mL
A1896701500 mL
Catalog number A1896702
Price (USD)
476.00
Each
Add to cart
Quantity:
10 x 500 mL
Price (USD)
476.00
Each
Add to cart

Gibco™ FluoroBrite™ DMEM features a background fluorescence that is comparable to PBS and 90% lower than that emitted by standard phenol red–free DMEM. Formulated to include the required nutrients for routine cell culture when supplemented with 10% fetal bovine serum and 4 mM L-glutamine or GlutaMAX™ Supplement, FluoroBrite™ DMEM is designed to enhance the signal-to-noise ratio of fluorophores, enabling researchers to visualize even the weakest fluorescent events in an environment that promotes optimum cell health. Additional features include:

• Enhancement of fluorescence signal during live-cell imaging
• DMEM-based to help preserve cell health

Live-cell fluorescence microscopy is an essential technique for the visualization of fundamentally important and physiologically relevant biological events. A key challenge with this technique is the ability to image weak fluorophores without causing cell damage, photobleaching, or undesirable changes to cell health. FluoroBrite™ DMEM helps address these issues.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Serum LevelStandard Serum Supplementation
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesHigh Glucose
Without AdditivesNo Glutamine, No Phenol Red, No HEPES, No Sodium Pyruvate
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Quantity10 x 500 mL
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C). Protect from light.

Frequently asked questions (FAQs)

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Should I be concerned about phenol red in my media when labeling my live cells with fluorescent dyes?

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.