Invitrogen

p38 MAPK alpha Polyclonal Antibody

p38 MAPK alpha Antibody in Immunocytochemistry (ICC/IF)

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p38 MAPK alpha Antibody (PA5-27831) in ICC/IF

Immunofluorescent analysis of p38 in paraformaldehyde-fixed HeLa cells using a p38 polyclonal antibody (Product # PA5-27831) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

p38 MAPK alpha Antibody in Western Blot (WB)

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Product Details

PA5-27831

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 227 of Human p38

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

AB_2545307

Product Specific Information

PA5-27831 targets p38 in IF and WB applications and shows reactivity with Human and mouse samples.

The PA5-27831 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 227 of Human p38.

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs. There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation. Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK. Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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