Invitrogen

ER Alpha Recombinant Protein, Ligand Binding Domain, GST Tag Recombinant Protein

Product Details

A15677

Applications

Tested Dilution

Publications

Nuclear Receptor Assay (NR)

Assay-dependent

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

282-595

Tag

GST-tag

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 8, with 20% glycerol, 0.02% NP-40, 5mM DTT, 0.5mM EDTA, 500mM KCl

Contains

no preservative

Storage conditions

-80°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

This Estrogen Receptor (ER) Alpha Ligand Binding Domain (LBD) is a 62.80 kDa recombinant human protein (amino acids 282-595) expressed as a GST fusion protein in baculovirus-infected insect cells. It was expressed and purified in the absence of exogenous ligands. ER Alpha-LBD recombinant protein binds [³H]-estradiol with sub-nM affinity in hydroxylapatite assays and binds specifically to estrogen response element (ERE) sequences.

ER Alpha-LBD recombinant protein is:
• Flexible—ideal for ligand binding and coregulator displacement assays developed in-house or partnered with our LanthaScreen™ ER Beta Coactivator Assay or LanthaScreen™ ER Alpha Competitive Binding kits
• Ready to use—active, purified, and tagged with a GST
• Convenient—available in bulk sizes

ER Alpha-LBD recombinant protein is manufactured under the strictest quality parameters. This product is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

Used in:
LanthaScreen™ TR-FRET ER Alpha Coactivator Assay Kit
LanthaScreen™ TR-FRET ER Alpha Competitive Binding Kit

For bulk reagents, email: !Mad-DiscoveryServices@lifetech.com

Protein Form: Recombinant, Ligand Binding Domain

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686N23123; ER; ER-alpha; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; hER-alpha36; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1