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Invitrogen
Human ACSL3 (aa 181-285) Control Fragment Recombinant Protein
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Product Details
RP-89591
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
MYNFQLVTLYATLGGPAIVHALNETEVTNIITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPHSKPLPSDIAVIM
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (91%), Rat (91%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-82427. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
An initial reaction in fatty acid metabolism in eukaryotic cells is activation of fatty acids catalyzed by acyl-CoA synthetase. FACL3 (fatty acid CoA ligase, long-chain 3) is identified as member of the acyl-CoA synthetase (ACS) family by PCR of rat brain cDNAs using primers based on the conserved region of the ACS protein. The 720-amino acid rat protein preferentially utilizes myristate, laurate, arachidonate, and eicosapentaenoate, and is expressed primarily in brain. The predicted 720-amino acid FACL3 human protein is 92% identical to that of rat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Arachidonate--CoA ligase; Fatty acid CoA ligase Acsl3; fatty-acid-Coenzyme A ligase, long-chain 3; LACS 3; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 3; Long-chain-fatty-acid--CoA ligase 3; Medium-chain acyl-CoA ligase Acsl3
Gene Aliases: ACS3; ACSL3; FACL3; LACS3; PRO2194
UniProt ID: (Human) O95573
Entrez Gene ID: (Human) 2181
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