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Invitrogen
Human ADPGK (aa 99-178) Control Fragment Recombinant Protein
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Product Details
RP-106863
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
SPGNGKDHSILHSRNDLEEAFIHFMGKGAAAERFFSDKETFHDIAQVASEFPGAQHYVGGNAALIGQKFAANSDLKVLLC
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (90%), Rat (90%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-66176. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The ADPGK gene encodes ADP-dependent glucokinase, an enzyme distinct from the ATP-dependent hexokinases as it employs adenosine diphosphate (ADP) rather than ATP to phosphorylate glucose to glucose-6-phosphate. This gene, initially identified in archaeal species, has been co-opted into mammalian systems where it plays roles primarily in glycolytic processes. Structurally, the mammalian ADPGK differs from its archaeal counterpart, exemplified by the rearrangement of its secondary structures, such as the alpha2 and alpha3 helices. These helices are replaced by an extended beta-strand region, indicating evolutionary adaptations for function in higher organisms like mice. Although its precise physiological role remains partially defined, ADPGK is notably expressed in both hematopoietic lineages and tumor tissues, suggesting potential significance in cancer biology and energy metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADP-dependent glucokinase; ADP-GK; ATP-dependent glucokinase; EC 2.7.1.147; RbBP-35
Gene Aliases: 2610017G09Rik; ADP-GK; ADPGK; PSEC0260
UniProt ID: (Human) Q9BRR6
Entrez Gene ID: (Human) 83440
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