Invitrogen

Human AKAP4 (aa 78-216) Control Fragment Recombinant Protein

Product Details

RP-92143

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

EIIVIKDTEKKDQSKTEGSVCLFKQAPSDPVSVLNWLLSDLQKYALGFQHALSPSTSTCKHKVGDTEGEYHRASSENCYSVYADQVNIDYLMNRPQNLRLEMTAAKNTNNNQSPSAPPAKPPSTQRAVISPDGECSIDD

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (81%), Rat (81%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-54129. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. AKAP4, also known as hAKAP82, is a major sperm fibrous sheath protein that localizes to the entire length of the flagellum of human sperm. It has been suggested that AKAP4 plays multiple roles in sperm motility and regulation of signal transduction pathways. AKAP4 has also been postulated to be a potential biomarker for breast cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A kinase (PRKA) anchor protein 4; A-kinase anchor protein 4; A-kinase anchor protein 82 kDa; AKAP 82; AKAP-4; AKAP4; cancer/testis antigen 99; HI; Major sperm fibrous sheath protein; PRKA4; protein kinase A anchoring protein 4; Protein kinase A-anchoring protein 4; testis-specific gene HI

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Gene Aliases: AKAP 82; AKAP-4; AKAP4; AKAP82; CT99; FSC1; hAKAP82; HI; p82; PRKA4