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Product Details
160-08-50UG
Product Specifications
Species
Human
Expression System
CHO cells
Amino acid sequence
TCPERALERR EEEANVVLTG TVEEILNVDP VQHTYSCKVR VWRYLKGKDL VARESLLDGG NKVVISGFGD PLICDNQVST GDTRIFFVNP APPYLWPAHK NELMLNSSLM RITLRNLEEV EFCVEDKPGT HFTPVPPTPP DACRGMLCGF GAVCEPNAEG PGRASCVCKK SPCPSVVAPV CGSDASTYSN ECELQRAQCS QQRRIRLLSR GPCGSRDPCS NVTCSFGSTC ARSADGLTAS CLCPATCRGA PEGTVCGSDG ADYPGECQLL RRACARQENV FKKFDGPCDP CQGALPDPSR SCRVNPRTRR PEMLLRPESC PARQAPVCGD DGVTYENDCV MGRSGAARGL LLQKVRSGQC QGRDQCPEPC RFNAVCLSRR GRPRCSCDRV TCDGAYRPVC AQDGRTYDSD CWRQQAECRQ QRAIPSKHQG PCDQAPSPCL GVQCAFGATC AVKNGQAACE CLQACSSLYD PVCGSDGVTY GSACELEATA CTLGREIQVA RKGPCDRCGQ CRFGALCEAE TGRCVCPSEC VALAQPVCGS DGHTYPSECM LHVHACTHQI SLHVASAGPC ETCGDAVCAF GAVCSAGQCV CPRCEHPPPG PVCGSDGVTY GSACELREAA CLQQTQIEEA RAGPCEQAEC GSGGSGSGED GDCEQELCRQ RGGIWDEDSE DGPCVCDFSC QSVPGSPVCG SDGVTYSTEC ELKKARCESQ RGLYVAAQGA CRGPTFAPLP PVAPLHCAQT PYGCCQDNIT AARGVGLAGC PSACQCNPHG SYGGTCDPAT GQCSCRPGVG GLRCDRCEPG FWNFRGIVTD GRSGCTPCSC DPQGAVRDDC EQMTGLCSCK PGVAGPKCGQ CPDGRALGPA GCEADASAPA TCAEMRCEFG ARCVEESGSA HCVCPMLTCP EANATKVCGS DGVTYGNECQ LKTIACRQGL QISIQSLGPC QEAVAPSTHP TSASVTVTTP GLLLSQALPA PPGALPLAPS STAHSQTTPP PSSRPRTTAS VPRTTVWPVL TVPPTAPSPA PSLVASAFGE SGSTDGSSDE ELSGDQEASG GGSGGLEPLE GSSVATPGPP VER
Molecular weight
111.9 kDa
Class
Recombinant
Type
Protein
Purity
≥ 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Determined by its ability to bind U-87 MG cells in a functional ELISA.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
Recombinant Human Agrin is 111.9 kDa protein containing 1073 amino acids, corresponding to the 110 kDa N-terminal subunit of the full length protein. As a result of glycosylation, Recombinant Human Agrin migrates with an apparent molecular mass of approximately 150-160 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis
Target Information
Agrin is a molecule that resides in the basal lamina of muscle cells and directs key events in post synaptic differentiation. Most notably, Agrin is responsible for the clustering of acetylcholine receptors (AChRs) on the cell surface and their localization to the neuromuscular junction. Several Agrin variants have been identified which arise from alternative mRNA splicings. Agrin splice forms having inserts at two sites in the carboxy terminus designated "y" and "z" display a high affinity for AChRs, while splice forms lacking these inserts associate with AChRs weakly. Muscle alpha-dystroglycan has been postulated to be the receptor for the clustering activity of agrin; however, this is a point of contention. Tyrosine phosphorylation has been implicated as a required early step in AChR aggregation. Interestingly, a unique receptor tyrosine kinase, designated MuSK, has been discovered that interacts with Agrin and is specifically localized to developing muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Agrin; agrin proteoglycan; RP23-139J18.2
Gene Aliases: AGRIN; AGRN; CMS8; CMSPPD
UniProt ID: (Human) O00468
Entrez Gene ID: (Human) 375790
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