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          • Proteins & Peptides ›
          • BAFF (BLyS) Proteins

          Invitrogen

          Human BAFF (BLyS) Recombinant Protein, eBioscience™

          View all (6) BAFF (BLyS) proteins

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          Cite Human BAFF (BLyS) Recombinant Protein, eBioscience™

          Product Details

          14-8943-80

          Applications
          Tested Dilution
          Publications

          Functional Assay (Functional)

          Assay-Dependent
          -

          Control (Ctrl)

          Assay-Dependent
          -
          Product Specifications

          Species

          Human

          Expression System

          E. coli

          Amino acid sequence

          Human BAFF, amino acids Ala134-Leu285 (Accession # Q9Y275)

          Molecular weight

          18.5 kDa

          Class

          Recombinant

          Type

          Protein

          Purity

          >90% by reducing SDS-PAGE

          Endotoxin concentration

          <0.40 ng/µg

          Activity

          ED50 = 1.6 µg/mL; determined by a mouse splenocyte survival assay.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.1 mg/mL

          Purification

          purified

          Storage buffer

          PBS

          Contains

          no preservative

          Storage conditions

          -80°C

          Shipping conditions

          Dry ice

          Product Specific Information

          Description: Recombinant human BAFF (B-cell activating factor), also referred to as BlyS, is a type II transmembrane protein of the TNF superfamily. BAFF is found on T cells, macrophages and dendritic cells. BAFF contains a cytoplasmic domain, transmembrane domain and extracellular domain which can be cleaved between amino acid 133 and 134 to produce a soluble form detectable in serum. Receptors for BAFF include TACI, BAMBI and BAFFR. BAFF/BLyS is a B lymphocyte stimulatory molecule; it induces B cell proliferation and immunoglobulin secretion. In addition BAFF can provide a co-stimulation signal to T lymphocytes. Elevated levels of BAFF has been implicated in the pathogenesis of some autoimmune diseases (particularly) B cell diseases such as systemic lupus erythaematosus. Human and mouse BAFF share 86% amino acid sequence identity.

          Applications Reported: Recombinant human BAFF is biologically active.

          Applications Tested: The ED50, as determined by a mouse splenocytes survival assay, is 1.6 µg/mL.

          Source: E. coli expressed amino acids Ala134-Leu285 accession # Q9Y275.

          Bioactivity: The ED50, as determined by a mouse splenocyte survival assay, is 1.6 µg/mL.

          Endotoxin: Less than 0.40 ng/ug as determined by the LAL assay. Purity: >90% as determined by reducing SDS-PAGE.

          Molecular Weight: 18.5 kDa.

          Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: ApoL related ligand TALL-1; B lymphocyte stimulator; B-cell-activating factor; B-lymphocyte stimulator; BAFF; BLyS; CD257; delta BAFF; Delta4 BAFF; Dendritic cell-derived TNF-like molecule; TALL-1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNF- and APOL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily member 13b; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; Tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor-like protein ZTNF4

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          Gene Aliases: BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF13B; TNFSF20; TNLG7A; UNQ401/PRO738; ZTNF4

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          UniProt ID: (Human) Q9Y275

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          Entrez Gene ID: (Human) 10673

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          It has to be done as per old AB suggested Products section.
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