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Invitrogen
Human BHLHB5 (aa 14-80) Control Fragment Recombinant Protein
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Product Details
RP-103577
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
EDDLFLHKSLSASTSKRLEAAFRSTPPGMDLSLAPPPRERPASSSSSPLGCFEPADPEGAGLLLPPP
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (91%), Rat (91%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-64189. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: basic helix-loop-helix domain containing, class B, 5; basic helix-loop-helix family, member e22; bHLHb5; bHLHe22; Class B basic helix-loop-helix protein 5; Class E basic helix-loop-helix protein 22; trinucleotide repeat containing 20; Trinucleotide repeat-containing gene 20 protein
Gene Aliases: Beta3; BHLHB5; BHLHE22; CAGL85; TNRC20
UniProt ID: (Human) Q8NFJ8
Entrez Gene ID: (Human) 27319
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