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Human BMP-7 Protein (PHC9541) in Functional
Recombinant human BMP-7 (Product # PHC9541) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-7 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-p... View More
Product Details
PHC9541
Applications
Tested Dilution
Publications
Product Specifications
Species
Human,
Mouse
Expression System
HEK293 cells
Molecular weight
38 kDa
Class
Recombinant
Type
Protein
Purity
>95% by SDS-PAGE
Endotoxin concentration
<0.5 EU/µg
Activity
ED50 = 8.31 ng/mL; determined by the dose-dependent induction of alkaline phosphatase production in ATDC-5 cells.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
10mM acetic acid
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Dry ice
Product Specific Information
Carrier-Free
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile water containing 0.1% human serum albumin. Apportion the reconstituted protein into working aliquots and store at ≤ -20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA.
Storage: Store the lyophilized protein at ≤ -20°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.
Target Information
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extra skeletal site. Based on its expression early in embryogenesis, BMP7 has a proposed role in early development. In addition, the fact that BMP7 is closely related to BMP5 and BMP6 has lead to speculation of possible bone inductive activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BMP; BMP-7; Bone morphogenetic protein; Bone morphogenetic protein 7; OP-1; Osteogenic protein 1; RP11-560A15.5
Gene Aliases: Bmp-7; BMP7; OP-1; OP1
UniProt ID: (Human) P18075, (Mouse) P23359
Entrez Gene ID: (Human) 655, (Mouse) 12162
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