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Invitrogen
Human BRUNOL5 (aa 347-393) Control Fragment Recombinant Protein
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Product Details
RP-101830
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
AQSPTVAETLHPAFSGVQQYTAMYPTAAITPIAHSVPQPPPLLQQQQ
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (91%), Rat (91%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-63842. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternatively spliced transcript variants have been identified in this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bruno-like 5; bruno-like 5 RNA binding protein; Bruno-like protein 5; CELF-5; CUG-BP and ETR-3 like factor 5; CUG-BP- and ETR-3-like factor 5; CUGBP Elav-like family member 5; RNA-binding protein BRUNOL-5
Gene Aliases: BRUNOL-5; BRUNOL5; CELF-5; CELF5
UniProt ID: (Human) Q8N6W0
Entrez Gene ID: (Human) 60680
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