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Invitrogen
Human CAPNS2 (aa 153-203) Control Fragment Recombinant Protein
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Product Details
RP-108284
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
KWQCVYKQYDRDHSGSLGSSQLRGALQAAGFQLNEQLYQMIVRRYANEDGD
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (86%), Rat (86%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibodies, PA5-84434, PA5-85009. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The Calpain family of proteins are calcium-regulated thiol proteases which have broad endopeptidase activity throughout the body. Calpain small subunit 2, also known as CSS2 or CAPNS2, is a calcium-dependent protease that is expressed ubiquitously in the cytoplasm. Part of a heterodimer composed of a small subunit and a large subunit, CSS2 catalyzes proteolysis of various proteins involved in cytoskeletal remodeling and signal transduction. CSS2 also acts as a chaperone to the larger subunit, mediating its correct folding and conformation. When bound as a heterodimer, CSS2 is thought to keep the catalytic activity of the large subunit dormant. After binding calcium, CSS2 is released from the complex, thereby activating the large subunit and allowing CSS2 to translocate from the cytoplasm to the cell membrane. Defects in the gene encoding CSS2 result in incorrect calpain activity and retarded fetal development, suggesting that CSS2 expression is essential for proper growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium-dependent protease small subunit 2; Calpain small subunit 2; calpain, small subunit 2; CSS2
Gene Aliases: CAPNS2
UniProt ID: (Human) Q96L46
Entrez Gene ID: (Human) 84290
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