Invitrogen

Human CD3 delta Fc Chimera Flag-tag Recombinant Protein

Human CD3 delta Fc Chimera Flag-tag Protein in SDS-PAGE (SDS-PAGE)

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Human CD3 delta Fc Chimera Flag-tag Protein (RP-88001) in SDS-PAGE

SDS-PAGE analysis of Human CD3 delta Fc Chimera Flag-tag (Product # RP-88001) shows purity to be: > 85 % (SDS-PAGE).

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Product Details

RP-88001

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human CD3 delta, amino acids Met1-Ala105 (Accession # NP_000723.1) with a C-terminal Flag-tagged human IgG1 Fc region

Tag

DYKDDDDK-tag, Fc-tag

Molecular weight

47-51 kDa

Class

Recombinant

Type

Protein

Purity

>85% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (1000 µL) be added to the vial to prepare a stock solution of 1 mg/mL.

Concentration is measured by UV.

Target Information

CD3d is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The CD3d protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in the CD3d gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms of CD3d have been found. Other variants of CD3d may also exist, but the full-length nature of their transcripts have yet to be defined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD3 antigen, delta subunit; CD3 delta; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); OKT3, delta chain; T-cell receptor T3 delta chain; T-cell surface glycoprotein CD3 delta chain