Invitrogen

Human CD38 His-tag Recombinant Protein

Human CD38 His-tag Protein in SDS-PAGE (SDS-PAGE)

FIGURE: 1 / 1

Human CD38 His-tag Protein (RP-87997) in SDS-PAGE

SDS-PAGE analysis of Human CD38 His-tag (Product # RP-87997). The specification for purity shows: > 97 % (SDS-PAGE), ≥ 95 % (SEC-HPLC).

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Product Details

RP-87997

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human CD38, amino acids Val43-Ile300 (Accession # NP_001766.2) with a C-terminal His-tag

Tag

His-tag

Molecular weight

43-45 kDa

Class

Recombinant

Type

Protein

Purity

>97% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Activity

1. Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDPribose.The specific activity is > 2,500 pmoles/min/ µg. 2. Measured by its binding ability in a functional ELISA. Immobilized Human CD38 His at 2 µg/ml (100 µL/well) can bind Mouse Anti-CD38 Antibody, the EC50 of Mouse Anti-CD38 Antibody is 30-120 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 1.25 mg/mL.

Concentration is measured by UV.

Target Information

CD38 is a multifunctional ectoenzyme and type II transmembrane glycoprotein involved in various cellular processes. It catalyzes the conversion of NAD into secondary messengers such as nicotinic acid adenine dinucleotide phosphate (NAADP) and cyclic ADP-ribose (cADPR). CD38 plays a crucial role in B cell development, with expression levels fluctuating from high in immature cells to low in intermediate ones, and back to high in mature B cells. It is also present in a variety of tissues and hematopoietic cells, including T cells, NK cells, and monocytes, and is used to phenotype leukemias and monitor HIV-1 progression. The CD34+CD38- population is considered to define the most pluripotent hematopoietic stem cells. In addition to its surface expression, CD38 has been identified in the nucleus, where it may regulate calcium levels. It functions as a multi-catalytic ectoenzyme, serving roles as an ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, and possibly NAD+ glycohydrolase, or as a cell surface receptor. CD38 is involved in the activation, proliferation, and differentiation of mature lymphocytes and mediates apoptosis of myeloid and lymphoid progenitor cells. It also participates in cell adhesion, signal transduction, and calcium signaling. Antibodies to CD38 are valuable for subtyping lymphomas and leukemias, detecting plasma cells (such as in myelomas), and marking activated B and T cells. CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma, and neuroblastoma. Dysfunctions in CD38 are associated with diseases like chronic lymphocytic leukemia and Richter's Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (p45); CD38 antigen p45; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; NAD(+) nucleosidase; NAD+ nucleosidase; T10