Invitrogen

Human CD96 His-tag Recombinant Protein

Human CD96 His-tag Protein in SDS-PAGE (SDS-PAGE)

FIGURE: 1 / 1

Human CD96 His-tag Protein (RP-88026) in SDS-PAGE

SDS-PAGE analysis of Human CD96 His-tag (Product # RP-88026). The specification for purity shows: ≥ 90 % (SDS-PAGE), ≥ 95 % (SEC-HPLC).

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Product Details

RP-88026

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human CD96 isoform 2, amino acids Met1-Met503 (Accession # P40200-2) with a C-terminal His-tag

Tag

His-tag

Molecular weight

120-130 kDa

Class

Recombinant

Type

Protein

Purity

>90% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD155/PVR hFc at 2 µg/ml (100 µL/well) can bind Human CD96 His, the EC50 of Human CD96 His is 200-800 ng/mL.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 2.5 mg/mL.

Concentration is measured by UV.

Target Information

CD96 is a cell adhesion receptor also known as TACTILE. A member of the Ig superfamily, CD96 is a single pass transmembrane protein consisting of three extracellular Ig-like domains and a proline/serine/threonine rich region. CD96 is expressed on resident monocytes and T cells at all developmental stages (including gamma-delta T cells, thymocytes, and NK cells). In the mouse, this receptor is not expressed on B cells. Unlike in humans, mouse CD96 does not exist as alternative splice variants. Reports indicate that CD96 binds CD155/poliovirus receptor and Nectin-1 to mediate NK cell adhesion to target cells and cytotoxicity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD96; Cell surface antigen CD96; DKFZp667E2122; sCD96; soluble CD96; T cell activation, increased late expression; T cell-activated increased late expression protein; T-cell surface protein tactile