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Invitrogen
Human CHKB (aa 282-357) Control Fragment Recombinant Protein
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Product Details
RP-92367
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
CEWVYDYTHEEWPFYKARPTDYPTQEQQLHFIRHYLAEAKKGETLSQEEQRKLEEDLLVEVSRYALASHFFWGLWS
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (84%), Rat (84%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-53847. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. Choline kinase, the initial enzyme in the sequence, plays a role in cell growth proliferation. A related protein, ChoKB (also, known as choline kinase beta), is a 395 amino acid enzyme that catalyzes the phosphorylation of choline by ATP in the presence of magnesium, thereby yielding phosphocholine and ADP. Like all choline kinases, ChoKB possesses ethanoalamine kinase activity and catalyzes the phosphorylation of ethanolamine. The gene encoding ChoKB is located less than 1 kb upstream of the CPT1B gene, suggesting that the ChoKB gene may regulate transcription CPT1B. In mice, mutations and/or deletions in the gene encoding ChoKB are the cause of hindlimb muscular dystrophy and neonatal bone deformity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Choline kinase beta; Choline kinase-like protein; Choline/ethanolamine kinase; choline/ethanolamine kinase beta; CK; CKEKB; EK; EKB; Ethanolamine kinase; Ethanolamine kinase beta
Gene Aliases: CHETK; CHKB; CHKL; CK; CKB; CKEKB; EK; EKB; MDCMC
UniProt ID: (Human) Q9Y259
Entrez Gene ID: (Human) 1120
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